Single-molecule DNA flexibility in the presence of base-pair mismatch
Single-molecule DNA flexibility in the presence of base-pair mismatch
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DOI：
10.1063/1.1997279
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发表时间：
2005-07
影响因子：
4
通讯作者：
K. Schallhorn;Katherine O. Freedman;Jessica M. Moore;Jun Lin;P. Ke
中科院分区：
文献类型：
--
作者：
K. Schallhorn;Katherine O. Freedman;Jessica M. Moore;Jun Lin;P. Ke
使用单对荧光共振能量转移技术表征了通过一端锚定在石英基底上的22个碱基对双链DNA的构象变化。碱基对错配是DNA损伤的主要形式，与完全匹配的DNA相比，碱基对错配为3和7的DNA分子两端的荧光供体和受体之间的能量传递分别降低了4.4%和10.4%。这一结果表明，破坏受损DNA中的氢键导致轮廓长度增加和柔韧性改善。
The conformational changes of a 22 base-pair double-stranded DNA, anchored via one end to a quartz substrate, have been characterized using a single-pair fluorescence resonance energy transfer technique. Base-pair mismatch, a major form of DNA damage, has been found to decrease the energy transfer between a fluorescence donor and an acceptor attached to the two ends of DNA molecules with 3 and 7 mismatches, by 4.4% and 10.4%, respectively, as compared to that for perfectly matched DNA. This result suggests that the disruption of the hydrogen bonds in damaged DNA leads to increased contour length and improved flexibility.