Development of a low-cost magnetic microfluidic chip for circulating tumour cell capture.
Development of a low-cost magnetic microfluidic chip for circulating tumour cell capture.
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DOI：
10.1049/iet-nbt.2011.0024
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发表时间：
2011-12
影响因子：
2.3
通讯作者：
Jingkai Xia;X. Chen;Chongzhi Zhou;Yao Li;Zhihai Peng
中科院分区：
文献类型：
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作者：
Jingkai Xia;X. Chen;Chongzhi Zhou;Yao Li;Zhihai Peng
作者开发了一种基于铁磁材料封装微柱（FMEMs）的选择性微磁激活细胞分选（MACS）芯片的新制造工艺，该工艺技术简单，成本低。FMEM产生高场梯度，以磁性吸引、捕获和保持其界面上的细胞。进行了系统测试模拟，以预测目标捕获的功效，并验证实际磁性粒子行为与模型预测吻合良好。为了确定新型microMACS芯片捕获循环肿瘤细胞（CTC）的能力，将SW620人结肠癌细胞用于体外流动模型系统中，并且能够以72.8%的效率捕获。CTC在芯片上的某个位置处的明显积累表明柱边界处的剪切应力事件可能影响功效，并且应在进一步优化工作中加以考虑。
The authors have developed a novel fabrication process for a selective micro-magnetic activated cell sorting (MACS) chip based on ferromagnetic material encapsulated micropillars (FMEMs), which is technically simple and low cost. The FMEM produces a high field gradient to magnetically attract, capture and hold cells on its interface. System test simulations were carried out to predict the efficacy of target capture and verify that the actual magnetic particles behaviour agreed well with model predictions. To determine the ability of the novel microMACS chip to capture circulating tumour cells (CTCs), SW620 human colon cancer cells were used in an in vitro flow model system and were able to be captured with the efficiency of 72.8%. The obvious accumulation of CTCs at a certain location on the chip suggested shear stress events at the pillar boundary may influence efficacy, and should be considered in further optimisation efforts.