The genome of camelpox virus
The genome of camelpox virus
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DOI：
10.1006/viro.2001.1343
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发表时间：
2002-03-30
期刊：
影响因子：
3.7
通讯作者：
Rock, DL
中科院分区：
文献类型：
--
作者：
Afonso, CL;Tulman, ER;Rock, DL
骆驼痘病毒（Camelpox virus，CMLV）是痘病毒科正痘病毒属的成员，是骆驼疾病的病原体。本文报道了CMLV的基因组序列并进行了分析。CMLV基因组全长205，719-bp，包含211个推定基因，由一个约7 kb的相同反向末端重复序列结合的中心区域组成。CMLV 017和CMLV 184之间的区域在基因顺序、基因含量和氨基酸组成方面具有高度相似性CMLV与痘苗病毒（VACV）的同源性平均为96%，表明CMLV与其他已知的正痘病毒（OPV）在结构和功能上有密切的关系。值得注意的是，CMLV包含一个约3 kb的独特区域，该区域编码三个ORF（CMLV 185，CMLV 186，CMLV 187），这些ORF与在其他脊索痘病毒属中发现的B22 R同源物最相似。在OPV中，CMLV与天花病毒（VARV）的关系最为密切，共享参与基本复制功能的所有基因和参与其他宿主相关功能的大部分基因。CMLV和VARV之间的差异包括大量基因的缺失和破坏。VARV中不存在27个CMLV ORF，包括NMDA样受体、磷脂酶D、Schlafen、MT-4毒力、kelch、VACV C8 L和牛痘（CPXV）B21 R蛋白的7个全长同源物。38个CMLV ORF，其中一些是较大基因的片段，与相应的VARV ORF大小相差超过10%（氨基酸）。基因组结构和所有ORF的DNA序列的系统发育分析表明，CMLV是清楚地不同于VARV和VACV，因为它已被建议为VARV，它可能起源于CPXV病毒样的祖先。(C)2002 Elsevier Science（美国）。
Camelpox virus (CMLV), a member of the Orthopoxvirus genus in the Poxviridae, is the etiologic agent of a disease of camels. Here we report the CMLV genomic sequence with analysis. The 205,719-bp CMLV genome contains 211 putative genes and consists of a central region bound by identical inverted terminal repeats of approximately 7 kb. A high degree of similarity in gene order, gene content, and amino acid composition in the region located between CMLV017 and CMLV184 (average 96% amino acid identity to vaccinia virus (VACV)) indicates a close structural and functional relationship between CMLV and other known orthopoxviruses (OPVs), Notably, CMLV contains a unique region of approximately 3 kb, which encodes three ORFs (CMLV185, CMLV186, CMLV187) absent in other OPVs, These ORFs are most similar to B22R homologues found in other chordopoxvirus genera. Among OPVs, CMLV is the most closely related to variola virus (VARV), sharing all genes involved in basic replicative functions and the majority of genes involved in other host-related functions. Differences between CMLV and VARV include deletion and disruption of a large number of genes. Twenty-seven CMLV ORFs are absent in VARV, including seven full-length homologues of NMDA-like receptor, phospholipase D, Schlafen, MT-4 virulence, kelch, VACV C8L, and cowpox (CPXV) B21R proteins. Thirty-eight CMLV ORFs, some of which are fragments of larger genes, differ in size from corresponding VARV ORFs by more than 10% (amino acids). Genome structure and phylogenetic analysis of DNA sequences for all ORFs indicate that CMLV is clearly distinct from VARV and VACV and, as it has been suggested for VARV, it may have originated from a CPXV virus-like ancestor. (C) 2002 Elsevier Science (USA).