Quantification of microRNA by gold nanoparticle probes
Quantification of microRNA by gold nanoparticle probes
复制标题
金纳米颗粒探针对 microRNA 的定量
DOI：
10.1016/j.ab.2008.02.003
复制
发表时间：
2008-05-15
影响因子：
2.9
通讯作者：
Xu, Shun-Qing
中科院分区：
文献类型：
--
作者：
Yang, Wen-Jie;Li, Xiao-Bo;Xu, Shun-Qing
MicroRNAs are important posttranscriptional regulators of gene expression in animals and plants. A sensitive and specific detection method is urgently needed for intensive studies on differential expression and regulatory roles of microRNA. Here we present a simple and reliable method for the quantification of microRNA. The hybridization products of target microRNA with capture probe and gold nanoparticle probe are immobilized onto the surface of a streptavidin-coated microplate, and the signal is amplified by silver enhancement. Distribution of miR-122a/miR-128 in mouse brain and liver tissue was detected by this method, and synthetic miRNA122a was quantified. This method allowed a lower detect limit of 10 fM with a linear dynamic range from 10 pM to 10 fM and a high specificity to discriminate one single oligonucleotide mismatch of the target microRNA. (C) 2008 Elsevier Inc. All rights reserved.
