Compartments in medulloblastoma with extensive nodularity are connected through differentiation along the granular precursor lineage.
Compartments in medulloblastoma with extensive nodularity are connected through differentiation along the granular precursor lineage.
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DOI：
10.1038/s41467-023-44117-x
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发表时间：
2024-01-08
影响因子：
16.6
通讯作者：
Pajtler, Kristian W.
中科院分区：
文献类型：
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作者：
Ghasemi, David R.;Okonechnikov, Konstantin;Rademacher, Anne;Tirier, Stephan;Maass, Kendra K.;Schumacher, Hanna;Joshi, Piyush;Gold, Maxwell P.;Sundheimer, Julia;Statz, Britta;Rifaioglu, Ahmet S.;Bauer, Katharina;Schumacher, Sabrina;Bortolomeazzi, Michele;Giangaspero, Felice;Ernst, Kati J.;Clifford, Steven C.;Saez-Rodriguez, Julio;Jones, David T. W.;Kawauchi, Daisuke;Fraenkel, Ernest;Mallm, Jan-Philipp;Rippe, Karsten;Korshunov, Andrey;Pfister, Stefan M.;Pajtler, Kristian W.
具有广泛结节性的髓母细胞瘤是小脑肿瘤，其特征在于两个不同的区室和可变的疾病进展。 MBEN 中控制增殖和分化之间平衡的机制仍知之甚少。在这里，我们采用多模式单细胞转录组分析来剖析这个过程。在结节间室中，我们发现了增殖的小脑颗粒神经元前体样恶性细胞，以及基质细胞、血管细胞和免疫细胞。相反，结节区室包含有丝分裂后、神经元分化的恶性细胞。两个区室通过中间细胞阶段连接，类似于主动迁移的 CGNP。值得注意的是，我们还发现了星形细胞样恶性细胞，发现于两个区室之间的过渡区的迁移和分化细胞附近。我们的研究揭示了空间组织组织及其与发育轨迹的联系，从而产生更良性的肿瘤表型。这种综合方法有望探索具有不同组织学的其他癌症的间室相互作用。广泛结节性髓母细胞瘤（MBEN）中调节增殖和分化平衡的机制仍知之甚少。在这里，单细胞多组学和空间分析描述了 MBEN 在发育轨迹背景下的空间组织组织。
Medulloblastomas with extensive nodularity are cerebellar tumors characterized by two distinct compartments and variable disease progression. The mechanisms governing the balance between proliferation and differentiation in MBEN remain poorly understood. Here, we employ a multi-modal single cell transcriptome analysis to dissect this process. In the internodular compartment, we identify proliferating cerebellar granular neuronal precursor-like malignant cells, along with stromal, vascular, and immune cells. In contrast, the nodular compartment comprises postmitotic, neuronally differentiated malignant cells. Both compartments are connected through an intermediate cell stage resembling actively migrating CGNPs. Notably, we also discover astrocytic-like malignant cells, found in proximity to migrating and differentiated cells at the transition zone between the two compartments. Our study sheds light on the spatial tissue organization and its link to the developmental trajectory, resulting in a more benign tumor phenotype. This integrative approach holds promise to explore intercompartmental interactions in other cancers with varying histology. The mechanisms regulating the balance between proliferation and differentiation in medulloblastomas with extensive nodularity (MBEN) remain poorly understood. Here, single cell multi-omics and spatial analysis characterises the spatial tissue organisation of MBEN in the context of the developmental trajectory.
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结节过度的髓母细胞瘤：放射学特征和病理相关性。
影响因子：
--
作者：
Yeh-Nayre LA;Malicki DM;Vinocur DN;Crawford JR
通讯作者：
Crawford JR
影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
64.8
作者：
Capper D;Jones DTW;Sill M;Hovestadt V;Schrimpf D;Sturm D;Koelsche C;Sahm F;Chavez L;Reuss DE;Kratz A;Wefers AK;Huang K;Pajtler KW;Schweizer L;Stichel D;Olar A;Engel NW;Lindenberg K;Harter PN;Braczynski AK;Plate KH;Dohmen H;Garvalov BK;Coras R;Hölsken A;Hewer E;Bewerunge-Hudler M;Schick M;Fischer R;Beschorner R;Schittenhelm J;Staszewski O;Wani K;Varlet P;Pages M;Temming P;Lohmann D;Selt F;Witt H;Milde T;Witt O;Aronica E;Giangaspero F;Rushing E;Scheurlen W;Geisenberger C;Rodriguez FJ;Becker A;Preusser M;Haberler C;Bjerkvig R;Cryan J;Farrell M;Deckert M;Hench J;Frank S;Serrano J;Kannan K;Tsirigos A;Brück W;Hofer S;Brehmer S;Seiz-Rosenhagen M;Hänggi D;Hans V;Rozsnoki S;Hansford JR;Kohlhof P;Kristensen BW;Lechner M;Lopes B;Mawrin C;Ketter R;Kulozik A;Khatib Z;Heppner F;Koch A;Jouvet A;Keohane C;Mühleisen H;Mueller W;Pohl U;Prinz M;Benner A;Zapatka M;Gottardo NG;Driever PH;Kramm CM;Müller HL;Rutkowski S;von Hoff K;Frühwald MC;Gnekow A;Fleischhack G;Tippelt S;Calaminus G;Monoranu CM;Perry A;Jones C;Jacques TS;Radlwimmer B;Gessi M;Pietsch T;Schramm J;Schackert G;Westphal M;Reifenberger G;Wesseling P;Weller M;Collins VP;Blümcke I;Bendszus M;Debus J;Huang A;Jabado N;Northcott PA;Paulus W;Gajjar A;Robinson GW;Taylor MD;Jaunmuktane Z;Ryzhova M;Platten M;Unterberg A;Wick W;Karajannis MA;Mittelbronn M;Acker T;Hartmann C;Aldape K;Schüller U;Buslei R;Lichter P;Kool M;Herold-Mende C;Ellison DW;Hasselblatt M;Snuderl M;Brandner S;Korshunov A;von Deimling A;Pfister SM
通讯作者：
Pfister SM
DOI：
10.1084/jem.20202350
发表时间：
2021-09-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guo D;Wang Y;Cheng Y;Liao S;Hu J;Du F;Xu G;Liu Y;Cai KQ;Cheung M;Wainwright BJ;Lu QR;Zhao Y;Yang ZJ
通讯作者：
Yang ZJ
影响因子：
16.6
作者：
通讯作者：
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