Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus.
Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus.
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DOI：
10.5941/myco.2014.42.3.296
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发表时间：
2014-09
期刊：
影响因子：
1.9
通讯作者：
Lee JS
中科院分区：
文献类型：
--
作者：
Jang IT;Hyun SH;Shin JW;Lee YH;Ji JH;Lee JS
我们从几种食用菌中选择平菇，因为它具有较高的抗痛风黄嘌呤氧化酶（XOD）抑制活性。用40℃蒸馏水处理平菇子实体48小时，XOD抑制剂提取量最大。所得XOD抑制剂经Sephadex G-50凝胶渗透层析、超滤、C18固相萃取层析和反相高效液相层析纯化，固收率为3%，其XOD抑制活性为0.9 mg/mL的IC50。纯化得到的XOD抑制剂为三肽，氨基酸序列为苯丙氨酸-半胱氨酸-组氨酸，分子量为441.3 Da。含XOD抑制剂的平耳菌超滤液在氧酸钾诱导痛风的Sprague-Dawley大鼠模型中显示出剂量依赖性的抗痛风作用，在500和1000 mg/kg剂量下显示出血清尿酸水平降低，尽管效果不如商用抗痛风剂别嘌呤醇在50 mg/kg剂量下达到的效果大。
We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus ostreatus fruiting body was treated with distilled water at 40℃ for 48 hr. The XOD inhibitor thus obtained was purified by Sephadex G-50 gel permeation chromatography, ultrafiltration, C18 solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with 3% of solid yield, and its XOD inhibitory activity was 0.9 mg/mL of IC50. The purified XOD inhibitor was a tripeptide with the amino acid sequence phenylalanine-cysteine-histidine and a molecular weight of 441.3 Da. The XOD inhibitor-containing ultrafiltrates from Pleurotus ostreatus demonstrated dose-dependent anti-gout effects in a Sprague-Dawley rat model of potassium oxonate-induced gout, as shown by decreased serum urated levels at doses of 500 and 1,000 mg/kg, although the effect was not as great as that achieved with the commercial anti-gout agent, allopurinol when administered at a dose of 50 mg/kg.