Detection of Rickettsial DNA in ixodid ticks recovered from dogs and cats in Japan
Detection of Rickettsial DNA in ixodid ticks recovered from dogs and cats in Japan
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DOI：
10.1292/jvms.67.1217
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发表时间：
2005-12-01
影响因子：
1.2
通讯作者：
Inokuma, H
中科院分区：
文献类型：
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作者：
Hiraoka, H;Shimada, Y;Inokuma, H
从日本Ell的1137只狗和133只猫中回收的蜱的DNA通过基于柠檬酸合酶基因（gltA）的PCR和部分核苷酸测序检测立克次体感染。在巢式PCR中，共有91个犬蜱样品和18个猫蜱样品显示出适当大小的单一条带。对102个样本成功进行了序列分析。日本立克次体或近缘立克次体的DNA。主要在日本西部的16个县的38个舔体中检测到菌株。另外33例来自北海道、冲绳等13个都道府县，被发现为瑞士立克次体或密切相关的菌株。在日本全国19个都道府县共检测到29个与赤立立克次体或密切相关菌株具有最高同源性的DNA。从从福冈的一只狗身上取出的血蜱中检测到加拿大立克次体样DNA，从北海道的一只狗身上取出的硬蜱中检测到“tarasevichiae立克次体”样DNA。
DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19 prefectures, widespread throughout Japan. Rickettsia canada-like DNA was detected from Haemaphysalis sp. removed from a dog in Fukuoka, and 'Candidatus Rickettsia, tarasevichiae'-like DNA was from Ixodes sp. removed from a dog in Hokkaido.