PARVALBUMIN-IMMUNOREACTIVE NEURONS IN THE RAT NEOSTRIATUM - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
PARVALBUMIN-IMMUNOREACTIVE NEURONS IN THE RAT NEOSTRIATUM - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1016/0006-8993(90)90002-s
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发表时间：
1990-12-17
期刊：
影响因子：
2.9
通讯作者：
HEIZMANN, CW
中科院分区：
文献类型：
--
作者：
KITA, H;KOSAKA, T;HEIZMANN, CW
用光镜和电镜观察了大鼠新纹状体内小清蛋白（PV）免疫反应神经元。 纹状体中的少数神经元对PV（一种钙结合蛋白）呈免疫反应性。 他们中的大多数也强烈的免疫反应谷氨酸脱羧酶，但阴性的NADPH-黄递酶活性。 光镜观察显示，含PV神经元胞体呈梭形或多边形，大小中等至较大。 树突近端光滑呈圆柱形，远端呈静脉曲张状。 细PV免疫反应纤维与大的终扣分布不均匀的纹状体。 电子显微镜观察发现，PV免疫反应阳性神经元的胞体有一个深深缩进的核与核仁，往往是一个核内杆。 这些是纹状体中间神经元的形态学特征。 两个相邻的PV免疫反应树突之间形成缝隙连接。 共观察到175个终扣与PV免疫反应阳性神经元的胞体和树突形成突触。 其中115个直径小（小于1 μ m），含有密集的圆形小泡，主要与树突形成不对称突触。 其余60个终扣与PV阳性神经元的胞体和树突形成对称性突触。 观察到有髓和无髓轴突与终扣。 PV免疫反应终扣直径为0.3-2 μ m，含有直径约35 nm的圆形或细长囊泡。 终扣与突触后靶点形成对称的突触。 在100个PV免疫反应阳性终扣中，有51个位于中型神经元的胞体和近端树突上，其中含有一个大的、圆形的、位于中央的核。 其他的则与不同大小的树突形成突触。 偶尔观察到，静脉曲张的树突无棘接触了大量的PV免疫反应终扣。 该研究表明，在纹状体中，PV选择性的免疫细胞化学染色GABA能中间神经元，并且GABA能中间神经元被纳入纹状体的前馈抑制回路中。
Parvalbumin (PV)-immunoreactive neurons in rat neostriatum were studied under light and electron microscopes. A small number of neurons in the striatum were immunoreactive for PV (a Ca-binding protein). Most of them were also strongly immunoreactive for glutamate decarboxylase but were negative for NADPH-diaphorase activity. Light microscopic analysis revealed that PV-containing neurons have somata with fusiform or polygonal shape and are medium to large in size. The dendrites were smooth and cylindrical at the proximal portion but were varicose at the distal portion. Thin PV-immunoreactive fibers with large boutons were unevenly distributed in the striatum. Electron microscopy revealed that the somata of PV-immunoreactive neurons had a deeply indented nucleus with a nucleolus and often an intranuclear rod. These are the morphological features reported for interneurons of the striatum. Gap junctions formed between two neighboring PV-immunoreactive dendrites. A total of 175 boutons forming synapses with somata and dendrites of PV-immunoreactive neurons were examined. Of these, 115 were small in diameter (less than 1-mu-m), contained densely packed round vesicles and formed asymmetrical synapses mainly with dendrites. The other 60 boutons formed symmetrical synapses with somata and dendrites of PV-immunoreactive neurons. Both myelinated and unmyelinated axons with boutons were observed. PV-immunoreactive boutons had a diameter of 0.3-2-mu-m and contained round or elongated vesicles which were about 35 nm in diameter. The boutons formed symmetrical synapses with postsynaptic targets. Of the 100 PV-immunoreactive boutons, 51 were found on somata and proximal dendrites of medium-sized neurons containing a large, round, centrally located nucleus. The others formed synapses with dendrites of various sizes. It was occasionally observed that varicose dendrites free of spines were contacted by a large number of PV-immunoreactive boutons. The study indicates that, in the striatum, immunocytochemistry for PV selectivity stains GABAergic interneurons and that the GABAergic interneurons are incorporated in a feed-forward inhibitory circuit of the striatum.