Laminar distributions of muscarinic acetylcholine, serotonin, GABA and opioid receptors in human posterior cingulate cortex.
Laminar distributions of muscarinic acetylcholine, serotonin, GABA and opioid receptors in human posterior cingulate cortex.
复制标题
人后扣带皮层中毒蕈碱乙酰胆碱、血清素、GABA 和阿片受体的层状分布。
DOI：
10.1016/0306-4522(90)90359-c
复制
发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Bird,ED
中科院分区：
文献类型：
--
作者：
Vogt,BA;Plager,MD;Crino,PB;Bird,ED
实验动物研究已经证明了一些特定的皮层传入和神经元上的受体定位。目前的研究人类后扣带皮层评估特定受体的层状分布和它们可能与神经元的成分。采用盖玻片放射自显影和单颗粒计数技术，然后进行异质性分析，其中确定每个配体的峰结合层和异质性指数。通过测定各层的特异性结合作为所有层中结合的百分比来计算指数。减去与绝对均匀分布的差异，即9层中每层11.1%，并将绝对层流差异相加以形成指数。超过15的高指数反映了新皮层中的异质结合模式。毒蕈碱乙酰胆碱，5-羟色胺，阿片类，GABA和β肾上腺素受体的配体的结合进行了评价。哌仑西平结合在第二层的区域23 a，但非常均匀的异质性指数为8.9。相比之下，氧化震颤素-M结合在层IIIc中具有峰，指数为16.4，而AF-DX 116结合在层IIIa-b中具有峰，指数为30.6。在5-羟色胺摄取和受体结合的配体中，帕罗西汀结合均匀分布在层I-III中，并且具有9.8的低异质性指数。酮色林结合也是均匀的，由于其指数为8.9，该模式与帕罗西汀几乎相同。与此相反，血清素和8-羟基-2-（二正丙基氨基）四氢化萘的结合在第二层达到峰值，并有非常高的指数分别为20.8和50.3，这表明只有一个有限的关联与帕罗西汀分布。最后，有三个层包含阿片样物质、GABA和β肾上腺素受体配体的结合峰。首先，Ia层具有强啡肽-A结合峰值，后者的指数为22.6。其次，Tyr-d-Ala-Gly-MePhe-Gly-ol和2-d-青霉胺-5-d-青霉胺-脑啡肽结合在第II层达到峰值，其指数分别为8.6和17.4。第三，蝇蕈醇和（-）-氰基吲哚酚结合在IIIa-b层达到峰值，指数分别为29.6和11.1。在实验动物研究的背景下，氧化震颤素-M和帕罗西汀结合的异质性可能分别与丘脑和中缝核的终止相关。虽然5-羟色胺受体与5-羟色胺摄取位点共分布，但降钙素1A受体与这些位点存在显著错配。最后，突触后受体优先在不同的层中达到峰值，包括Ia层中的κ阿片受体，II层中的M1乙酰胆碱、降钙素1A、μ和δ阿片受体，IIIa-b层中的GABA A和β肾上腺素受体以及IIIc层中的降钙素2受体。因此，配体结合分析为人类大脑皮层的结构和连接提供了新的见解。
Experimental animal studies have demonstrated a number of receptor localizations on specific cortical afferents and neurons. The present study of human posterior cingulate cortex evaluates the laminar distributions of particular receptors and their likely association with components of the neuropil. Coverslip autoradiographic and single grain counting techniques were used followed by heterogeneity analysis in which the layer of peak binding and an index of heterogeneity were determined for each ligand. The index was calculated by determining specific binding by layer as a percentage of binding in all layers. The differences from an absolutely homogeneous distribution, i.e. 11.1% for each of nine layers, were subtracted and the absolute laminar differences summed to form the index. High indices of over 15 reflected heterogeneous binding patterns in neocortex. The binding of ligands for muscarinic acetylcholine, serotonin, opioid, GABA and beta adrenoceptors was evaluated.Pirenzepine binding peaked in layer II of area 23a but was extremely homogeneous with an index of heterogeneity of 8.9. In contrast, oxotremorine-M binding had a peak in layer IIIc and an index of 16.4, while AF-DX 116 binding peaked in layer IIIa-b and had an index of 30.6. Of the ligands for serotonin uptake and receptor binding paroxetine binding was evenly distributed in layers I–III and had a low index of heterogeneity of 9.8. Ketanserin binding was also homogeneous and, since it had an index of 8.9, this pattern was virtually the same as that for paroxetine. In contrast, serotonin and 8-hydroxy-2-(di-n-propylamino)tetralin binding peaked in layer II and had very high indices of 20.8 and 50.3, respectively, suggesting only a limited association with that of the paroxetine distribution. Finally, there were three layers which contained peaks in binding for ligands for opioid, GABA and beta adrenoceptors. Firstly, layer Ia had peak dynorphin-A binding, the latter of which had an index of 22.6. Secondly, Tyr-d-Ala-Gly-MePhe-Gly-ol and 2-d-penicillamine-5-d-penicillamine-enkephalin binding peaked in layer II and had indices of 8.6 and 17.4, respectively. Thirdly, muscimol and (−)-cyanopindolol binding peaked in layer IIIa-b and had indices of 29.6 and 11.1, respectively.When viewed in the context of experimental animal studies, it is likely that heterogeneities in oxotremorine-M and paroxetine binding are associated with the termination of the thalamic and raphe nuclei, respectively. While serotonin, receptors are co-distributed with serotonin uptake sites, serotonin1Areceptors have a significant mismatch with these sites. Finally, postsynaptic receptors preferentially peak in different layers including kappa opioid receptors in layer Ia, M1acetylcholine, serotonin1A, mu and delta opioid receptors in layer II, GABAAand beta adrenoceptors in layer IIIa-b and serotonin2receptors in layer IIIc. Thus, ligand binding analyses provide new insights into the structure and connections of human cerebral cortex.