The diploid genome sequence of Candida albicans
The diploid genome sequence of Candida albicans
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DOI：
10.1073/pnas.0401648101
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发表时间：
2004-05-11
影响因子：
11.1
通讯作者：
Scherer, S
中科院分区：
文献类型：
--
作者：
Jones, T;Federspiel, NA;Scherer, S
我们目前的二倍体基因组序列的真菌病原体白色念珠菌。由于白色念珠菌没有已知的单倍体或纯合形式，因此在菌株SC 5314中作为杂合二倍体基因组的全基因组鸟枪法进行测序，该菌株是广泛用于分子分析的菌株的亲本的临床分离株。我们开发了计算方法来组装一个二倍体基因组序列，与现有的物理作图数据非常一致。我们提供了生物体杂合性的全基因组描述。比较基因组分析提供了重要的线索，物种的进化及其发病机制。
We present the diploid genome sequence of the fungal pathogen Candida albicans. Because C albicans has no known haploid or homozygous form, sequencing was performed as a whole-genome shotgun of the heterozygous diploid genome in strain SC5314, a clinical isolate that is the parent of strains widely used for molecular analysis. We developed computational methods to assemble a diploid genome sequence in good agreement with available physical mapping data. We provide a whole-genome description of heterozygosity in the organism. Comparative genomic analyses provide important clues about the evolution of the species and its mechanisms of pathogenesis.