Relationship between interleukin-6 polymorphism and susceptibility to chronic hepatitis B virus infection.
Relationship between interleukin-6 polymorphism and susceptibility to chronic hepatitis B virus infection.
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DOI：
10.3748/wjg.v19.i40.6888
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发表时间：
2013-10
影响因子：
4.3
通讯作者：
Xiao-miao Zhao;Yu-feng Gao;Qin Zhou;F. Pan;Xu Li
中科院分区：
文献类型：
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作者：
Xiao-miao Zhao;Yu-feng Gao;Qin Zhou;F. Pan;Xu Li
目的探讨白介素6（IL-6）基因标签单核苷酸多态性（标签SNP）与中国汉族人群慢性乙型肝炎病毒（HBV）感染易感性的关系。方法 我们对 501 名中国慢性 HBV 感染者和 301 名自限性 HBV 感染者作为对照进行了病例对照研究。使用酚/氯仿和 MaXtract 高密度管从所有受试者的全血中分离基因组 DNA。使用第二阶段 HapMap 项目小组（北京汉族）的基因型数据鉴定标签 SNP。通过多重快照技术对 IL-6 中的四个标签 SNP（rs17147230A/T、rs2066992G/T、rs2069837A/G 和 rs2069852A/G）进行基因分型。计算并分析基因型和等位基因频率。结果 5个单倍型均进入分析，频率均大于0.03。其中一种单倍型 TTAA 在两组之间存在显着差异。总体单倍型 P 值为：ATAA，P = 0.605，OR (95%CI) = 1.056 (0.860-1.297)； TGAG，P = 0.385，OR (95%CI) = 1.179 (0.813-1.709)； TGGG，P = 0.549，OR (95%CI) = 1.087 (0.827-1.429)； TTAA，P = 0.004，OR (95%CI) = 0.655 (0.491-0.873)； TTAG，P = 0.266，或 (95%CI) = 1.272 (0.832-1.944)。然而，这四个 SNP 与慢性 HBV 感染的易感性没有显着的基因型/等位基因关联。总体等位基因 P 值为：rs17147230，P = 0.696，OR (95%CI) = 1.041 (0.850-1.276)； rs2066992，P = 0.460，OR (95%CI) = 1.090 (0.868-1.369)； rs2069837，P = 0.898，OR (95%CI) = 0.983 (0.759-1.274)； rs2069852，P = 0.165，OR (95%CI) = 0.859 (0.693-1.064)。总体基因型P值为：rs17147230，P=0.625； rs2066992，P = 0.500； rs2069837，P = 0.853；和 rs2069852，P = 0.380。结论 IL-6基因的4个标签SNPs可能与中国汉族人群慢性乙型肝炎病毒感染的易感性相关。
AIM To identify the relationship between tag single nucleotide polymorphisms (tag SNPs) of interleukin-6 (IL-6) gene and susceptibility to chronic hepatitis B virus (HBV) infection in a Han Chinese population. METHODS We performed a case-control study of 501 Chinese patients with chronic HBV infection and 301 self-limiting HBV-infected individuals as controls. Genomic DNA was isolated from the whole blood of all subjects using phenol/chloroform with MaXtract high-density tubes. Tag SNPs were identified using genotype data from the panel (Han Chinese in Beijing) of the phase II HapMap Project. Four tag SNPs in IL-6 (rs17147230A/T, rs2066992G/T, rs2069837A/G and rs2069852A/G) were genotyped by the Multiplex Snapshot technique. The genotype and allele frequencies were calculated and analyzed. RESULTS Five haplotypes were involved in the analysis, with frequencies higher than 0.03. One of the haplotypes, TTAA, was significantly different between the two groups. Overall haplotype P values were: ATAA, P = 0.605, OR (95%CI) = 1.056 (0.860-1.297); TGAG, P = 0.385, OR (95%CI) = 1.179 (0.813-1.709); TGGG, P = 0.549, OR (95%CI) = 1.087 (0.827-1.429); TTAA, P = 0.004, OR (95%CI) = 0.655 (0.491-0.873); TTAG, P = 0.266, OR (95%CI) = 1.272 (0.832-1.944). However, the four SNPs showed no significant genotype/allele associations with susceptibility to chronic HBV infection. Overall allele P values were: rs17147230, P = 0.696, OR (95%CI) = 1.041 (0.850-1.276); rs2066992, P = 0.460, OR (95%CI) = 1.090 (0.868-1.369); rs2069837, P = 0.898, OR (95%CI) = 0.983 (0.759-1.274); rs2069852, P = 0.165, OR (95%CI) = 0.859 (0.693-1.064). Overall genotype P values were: rs17147230, P = 0.625; rs2066992, P = 0.500; rs2069837, P = 0.853; and rs2069852, P = 0.380. CONCLUSION The four tag SNPs of IL-6 gene may be associated with susceptibility to chronic HBV infection in the Han Chinese population.