INCORPORATION OF NEUTRAL RED AND ACRIDINE ORANGE INTO DEVELOPING POLIOVIRUS PARTICLES MAKING THEM PHOTOSENSITIVE
INCORPORATION OF NEUTRAL RED AND ACRIDINE ORANGE INTO DEVELOPING POLIOVIRUS PARTICLES MAKING THEM PHOTOSENSITIVE
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DOI：
10.1016/0042-6822(61)90127-1
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发表时间：
1961-01-01
期刊：
影响因子：
3.7
通讯作者：
MELNICK, JL
中科院分区：
文献类型：
--
作者：
CROWTHER, D;MELNICK, JL
本研究关注中性红、吖啶橙和光照对猴肾细胞培养物中强毒（马奥尼株）和减毒（LSc株）脊髓灰质炎病毒生长的影响。在无细胞的情况下，成熟的、有感染性的病毒与染料一起孵育1小时，然后暴露于白光下，其滴度没有降低。在含有中性红或吖啶橙的细胞中生长的病毒随后可能会被光照灭活，这表明染料掺入到了正在发育的病毒中。中性红和光照对强毒株和减毒株的作用相同。还讨论了醌亚胺染料（中性红、甲苯胺蓝）和吖啶类（吖啶橙、原黄素）之间的生物学和化学相似性。
This study concerns the effects of neutral red, acridine orange, and light on the growth of virulent (Mahoney) and attenuated (LSc) polioviruses in monkey kidney cell cultures. Mature, infective virus incubated with the dyes for 1 hour in the absence of cells and then exposed to white light showed no reduction in titer. Virus grown in cells containing neutral red or acridine orange may be subsequently inactivated by light, thus indicating an incorporation of the dye into the developing virus. The effect of neutral red and light was the same for the virulent and the attenuated strains. The biological and chemical similarities between the quinonimide dyes (neutral red, toluidine blue) and the acridines (acridine orange, proflavine) are discussed.