Contractile protein expression in bladder smooth muscle is a marker of phenotypic modulation after outlet obstruction in the rabbit model
Contractile protein expression in bladder smooth muscle is a marker of phenotypic modulation after outlet obstruction in the rabbit model
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DOI：
10.1016/s0022-5347(05)66585-8
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发表时间：
2001-03-01
影响因子：
6.6
通讯作者：
McConnell, JD
中科院分区：
文献类型：
--
作者：
Burkhard, FC;Lemack, GE;McConnell, JD
目的：我们确定了兔模型部分膀胱出口梗阻缓解前后收缩蛋白表达的变化，并评估了它们作为恢复预测因素的潜在作用。材料和方法：我们检查了平滑肌肌球蛋白重链亚型SM 2与SM 1的比例、钙调蛋白亚型表达和梗阻和通畅的成年兔膀胱的膀胱功能。膀胱测压、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹分析，以确定膀胱功能和收缩蛋白表达的变化。
Purpose: We determined changes in contractile protein expression before and after the relief of partial bladder outlet obstruction in the rabbit model and assessed their potential role as predictors of recovery.Materials and Methods: We examined the ratio of the smooth muscle myosin heavy chain isoforms SM2-to-SM1, caldesmon isoform expression and bladder function in obstructed and unobstructed adult rabbit bladders. Cystometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis were done to determine changes in bladder function and contractile protein expression.Results: Overall we observed significant correlation of bladder weight with the SM2-to-SM1 ratio (p