A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals
A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals
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DOI：
10.1111/j.1365-313x.2005.02377.x
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发表时间：
2005-05-01
期刊：
影响因子：
7.2
通讯作者：
Salomon, S
中科院分区：
文献类型：
--
作者：
Voigt, CA;Schäfer, W;Salomon, S
禾谷镰刀菌（Fusarium graminearum）是小麦赤霉病（Fusarium head blight，FHB）的病原菌，是造成小麦和玉米等粮食作物严重减产的重要病原菌。与其他真菌病原体一样，F.禾谷镰刀菌分泌各种胞外酶，推测这些酶参与宿主感染。F. graminearum强烈诱导培养小麦胚芽油，这使我们能够分离，克隆和表征基因（FGL 1）编码的分泌脂肪酶。表达分析表明，FGL 1是诱导含脂底物和抑制葡萄糖。在植物中，FGL 1的转录检测后1天的小麦穗感染。通过特异性证明在异源宿主中表达后的脂肪酶活性来验证FGL 1基因产物的功能。已知的脂肪酶抑制剂Ebelactone B抑制该酶的脂解活性。当野生型分生孢子补充乙内酯B时，疾病严重程度大大降低。转化介导的FGL 1的破坏导致降低细胞外脂肪分解活性的文化和降低的毒力小麦和玉米。
Fusarium graminearum is the causal agent of the Fusarium head blight (FHB) and a destructive pathogen of cereals accounting for high grain yield losses especially on wheat and maize. Like other fungal pathogens, F. graminearum secretes various extracellular enzymes, which are hypothesized to be involved in host infection. Extracellular lipolytic activity of F. graminearum was strongly induced in culture by wheat germ oil; this allowed us to isolate, clone, and characterize a gene (FGL1) encoding a secreted lipase. Expression analysis indicated that FGL1 is induced by lipid-containing substrates and repressed by glucose. In planta, FGL1 transcription was detected 1 day post-infection of wheat spikes. The function of the FGL1 gene product was verified by specifically demonstrating lipase activity after expression in a heterologous host. Ebelactone B, a known lipase inhibitor, repressed the lipolytic activity of the enzyme. Disease severity was strongly reduced when wild-type conidia were supplemented with ebelactone B. Transformation-mediated disruption of FGL1 led to reduced extracellular lipolytic activity in culture and to reduced virulence to both wheat and maize.