Organic acids prevent aluminum-induced conformational changes in calmodulin.
Organic acids prevent aluminum-induced conformational changes in calmodulin.
复制标题
有机酸可防止铝诱导的钙调蛋白构象变化。
DOI：
10.1016/0006-291x(84)91662-0
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Haug,A
中科院分区：
文献类型：
--
作者：
Suhayda,CG;Haug,A
当[柠檬酸盐]/[钙调蛋白]摩尔比超过10：1时，柠檬酸盐可以阻止铝与钙调蛋白结合，当以微摩尔浓度存在于蛋白质溶液中时，如通过荧光和圆二色光谱法所测定的。相比之下，一旦铝-钙调蛋白复合物（3：1）形成，柠檬酸盐仅部分有效地将钙调蛋白恢复至其天然结构，如通过蛋白质的α-螺旋含量所测量的。考虑到柠檬酸盐-铝螯合物的稳定常数的大小，柠檬酸盐和可能的其他羧酸可以保护钙调蛋白，从而保护细胞免受有毒铝离子的伤害。
At a molar excess of 10:1 for [citrate]/[calmodulin], citrate can prevent aluminum binding to calmodulin when present in the protein solution in micromolar concentration, as determined by fluorescence and circular dichroism spectroscopy. In contrast, citrate is only partially effective in restoring calmodulin to its native structure once the aluminum-calmodulin complex (3:1) is formed, as measured by the α-helix content of the protein. Considering the magnitude of the stability constant of the citrate-aluminum chelate, citrate and perhaps other carboxylic acids may protect calmodulin, and thus cells, from toxic aluminum ions.