Glutamine auxotrophs with mutations in a nitrogen regulatory gene, ntrC, that is near glnA.
Glutamine auxotrophs with mutations in a nitrogen regulatory gene, ntrC, that is near glnA.
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谷氨酰胺营养缺陷型，氮调节基因 NTrC（靠近 glnA）发生突变。
DOI：
10.1007/bf00270646
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Kustu,S
中科院分区：
文献类型：
--
作者：
Wei,GR;Kustu,S
Some mutations to glutamine auxotrophy in the 86 unit region of theSalmonellachromosome lie within thenitrogen regulatory gene,ntrC, rather than the structural gene encoding glutamine synthetase,glnA. Assignment of mutations tontrCis based on fine structure mapping by P22-mediated transduction and on complementation analysis. Strains withntrClesions that cause glutamine auxotrophy (NtrCrepressor) have very low levels of glutamine synthetase (lower than those of strains that completely lackntrCfunction and comparable to those of strains that lackatrAfunction). NtrCrepstrains fail to increase synthesis of glutamine synthetase or several amino acid transport components under nitrogen limiting conditions. Thus, likentrAstrains, they appear to repressglnAtranscription and fail to activate transcription ofglnAor other nitrogen controlled genes. Mutations that suppress the glutamine requirement caused by NtrCreplesions arise at high frequency; these mutations also suppress the glutamine requirement caused byntrAlesions. Several suppressor mutations result in loss of function ofntrC.
