Role of red blood cell lysis and iron in hydrocephalus after intraventricular hemorrhage
Role of red blood cell lysis and iron in hydrocephalus after intraventricular hemorrhage
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DOI：
10.1038/jcbfm.2014.56
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发表时间：
2014-06-01
影响因子：
6.3
通讯作者：
Xi, Guohua
中科院分区：
文献类型：
--
作者：
Gao, Chao;Du, Hanjian;Xi, Guohua
凝血酶和铁是脑内出血诱导的脑损伤的两个主要参与者，我们最近的研究发现凝血酶有助于脑积水的发展在大鼠模型的脑室出血（IVH）。本研究探讨了红细胞溶解和铁在IVH后脑积水中的作用。本研究分为三个部分。首先，雄性Sprague-Dawley大鼠接受盐水、包装或裂解的RBC注射到右侧脑室中。第二，大鼠脑室内注射铁或生理盐水。第三，大鼠接受与去铁胺（0.5mg在5 μ L盐水中）或盐水混合的裂解的RBC的脑室内注射。所有大鼠在24小时时进行磁共振成像，然后安乐死以进行脑水肿测量、蛋白质印迹分析或脑组织学检查。我们发现，脑室注射溶解的红细胞，而不是包装的红细胞，导致脑室扩大和脑血红素氧合酶-1和铁蛋白在24小时显着增加。脑室注射铁24小时后也导致心室扩大和室壁损伤。同时注射去铁胺可减少溶红细胞所致的心室扩大（P
Thrombin and iron are two major players in intracerebral hemorrhage-induced brain injury and our recent study found that thrombin contributes to hydrocephalus development in a rat model of intraventricular hemorrhage (IVH). This study investigated the role of red blood cell (RBC) lysis and iron in hydrocephalus after IVH. There were three parts to this study. First, male Sprague-Dawley rats received an injection of saline, packed, or lysed RBCs into the right lateral ventricle. Second, rats had an intraventricular injection of iron or saline. Third, the rats received intraventricular injection of lysed RBCs mixed with deferoxamine (0.5 mg in 5 mu L saline) or saline. All rats underwent magnetic resonance imaging at 24 hours and were then euthanized for brain edema measurement, western blot analysis, or brain histology. We found that intraventricular injection of lysed RBCs, but not packed RBCs, resulted in ventricular enlargement and marked increases in brain heme oxygenase-1 and ferritin at 24 hours. Intraventricular injection of iron also resulted in ventricular enlargement and ventricular wall damage 24 hours later. Coinjection of deferoxamine reduced lysed RBC-induced ventricular enlargement (P