Design a novel asymmetric bifurcating microfluidic channel for cell separation by size
Design a novel asymmetric bifurcating microfluidic channel for cell separation by size
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设计一种新型不对称分叉微流体通道，用于按尺寸分离细胞
DOI：
10.1109/ascc.2015.7244477
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M Umezu
中科院分区：
文献类型：
--
作者：
Z Feng;T Nakamura;D Sato;T Kitajima;M Umezu
在这篇论文中，我们展示了一种新的非对称分叉微通道，用于按大小分离细胞。在开发这种微通道的过程中，我们提出了设计和优化准则，包括基于Segre-Silberberg效应分析的进出口设计和使分叉处的迁移力尽可能大的优化原则。利用大鼠成纤维细胞和红细胞的实验验证了这些设计原则，并用最优设计验证了分离功能。微通道的简单结构使得在1.0×1.0mm2的空间内串联几个这样的微通道成为可能，并且实现了高达10~3∼~104倍的富集率。
In this paper, we exhibit a novel asymmetric bifurcating microchannel for cell separation by size. As developing this microchannel, we proposed the design and optimization criteria, which included the design of inlet and outlet based on the analysis of the Segre-Silberberg effect and the optimization principle to render the migration force at the bifurcation portion as great as possible. Experiments using rat fibroblasts and red blood cells verified these design principles and confirmed the separation function with the optimal design. The simple configuration of the microchannel makes it feasible to serialize several such microchannels within 1.0×1.0 mm2space and to achieve enrichment rate as high as 103∼104folds.