Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers
Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers
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DOI：
10.1016/j.archoralbio.2016.10.003
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Souza, Margareth Maria
中科院分区：
文献类型：
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作者：
Lima, Rodrigo Lopes;Holanda-Afonso, Rosenilde Carvalho;Souza, Margareth Maria
目的：本研究通过分析胚胎、间充质和神经干细胞表面标志物的表达来鉴定和表征牙囊干细胞（DFSC）。设计使用胚胎干细胞标记物(OCT4和SOX2)、间充质干细胞(MSC)标记物(Notch1、活性Notch1、STRO、CD44、HLA-ABC、CD90)、神经干细胞标记物(巢蛋白和(β-III-微管蛋白)、神经嵴干细胞(NCSC)标记物(p75和HNK1)和神经胶质细胞标记物通过免疫细胞化学评估牙囊细胞(DFC)进行RT-PCR来鉴定DFC和牙囊组织中OCT4和NANOG的表达。结果：免疫细胞化学和RT-PCR分析显示，很大比例的DFC5表达人胚胎干细胞标记物OCT4（75%），而相当数量的MSC（90%）表达Notch1、STRO、CD44和HLA-ABC。此外，可以证明牙囊含有显着比例的神经干/祖细胞，表达 p-III-微管蛋白 (90%) 和巢蛋白 (70%)。有趣的是，免疫细胞化学显示 DFC5 对 p75 (50%)、HNK1 呈阳性。
Objective: This study was conducted to identify and characterize dental follicle stem cells (DFSCs) by analyzing expression of embryonic, mesenchymal and neural stem cells surface markers. Design Dental follicle cells (DFCs) were evaluated by immunocytochemistry using embryonic stem cells markers (OCT4 and SOX2), mesenchmal stem cells (MSCs) markers (Notchl, active Notchl, STRO, CD44, HLA-ABC, CD90), neural stem cells markers (Nestin and (beta-III-tubulin), neural crest stem cells (NCSCs) markers (p75 and HNK1) and a glial cells marker (GFAP). RT-PCR was performed to identify the expression of OCT4 and NANOG in DFCs and dental follicle tissue.Results: Immunocytochemistry and RT-PCR analysis revealed that a significant proportion of the DFC5 evaluated expressed human embryonic stem cells marker OCT4 (75%) whereas NANOG was weakly expressed. A considerable amount of MSCs (90%) expressed Notchl, STRO, CD44 and HLA-ABC. However, they were weakly positive for CD90. Moreover, it was possible to demonstrate that dental follicle contains a significant proportion of neural stem/progenitors cells, expressing p-III-tubulin (90%) and nestin (70%). Interestingly, immunocytochemistry showed DFC5 positive for p75 (50%), HNK1 (