HOST-RESISTANCE TO A FUNGAL TOMATO PATHOGEN LOST BY A SINGLE BASE-PAIR CHANGE IN AN AVIRULENCE GENE
HOST-RESISTANCE TO A FUNGAL TOMATO PATHOGEN LOST BY A SINGLE BASE-PAIR CHANGE IN AN AVIRULENCE GENE
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DOI：
10.1038/367384a0
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发表时间：
1994-01-27
期刊：
影响因子：
64.8
通讯作者：
DEWIT, PJGM
中科院分区：
文献类型：
--
作者：
JOOSTEN, MHAJ;COZIJNSEN, TJ;DEWIT, PJGM
在大多数情况下，活体营养型病原体和植物之间的相互作用中的宿主基因型特异性符合基因对基因模型1;感染的成功或失败取决于病原体和宿主植物中互补基因、无毒基因和抗性基因的存在或缺失。通过诱导宿主的过敏反应来表达的抗性被设想为基于病原体的识别，通过病原体无毒基因的产物（所谓的种族特异性激发子）和宿主植物中的受体（抗性基因的推定产物）之间的直接相互作用来介导。生物营养型真菌Cladosporium fulvum和它的唯一宿主番茄之间的相互作用是研究真菌-植物基因间关系的一个成熟的模型系统。在这里，我们报告了C.及其编码无毒基因的克隆。我们提出的证据表明，在自然界中，一个单一的碱基对的变化，这个无毒基因导致以前无毒的番茄基因型携带互补的CF 4抗性基因的种族的毒力。
HOST genotype specificity in interactions between biotrophic pathogens and plants in most cases complies with the gene-for-gene model1; success or failure of infection is determined by absence or presence of complementary genes, avirulence and resistance genes, in the pathogen and host plant, respectively. Resistance, expressed by the induction of a hypersensitive response in the host, is envisaged to be based on recognition of the pathogen, mediated through direct interaction between products of pathogen avirulence genes (the so-called race-specific elicitors) and receptors in the host plant, the putative products of resistance genes1. The interaction between the biotrophic fungus Cladosporium fulvum and its only host, tomato (Lycopersicon esculentum), is a well-established model system for studying fungus-plant gene-for-gene relationships1. Here we report the isolation of race-specific elicitor AVR4 of C. fulvum and the cloning of its encoding avirulence gene. We present evidence that, in nature, a single base-pair change in this avirulence gene leads to virulence of races previously avirulent on tomato genotypes carrying the complementary Cf4 resistance gene.