INFLUENCE OF THE PH OF THE EXTRACTION MEDIUM ON THE COMPOSITION OF BIRCH (BETULA-VERRUCOSA) POLLEN EXTRACTS
INFLUENCE OF THE PH OF THE EXTRACTION MEDIUM ON THE COMPOSITION OF BIRCH (BETULA-VERRUCOSA) POLLEN EXTRACTS
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DOI：
10.1111/j.1398-9995.1995.tb01173.x
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发表时间：
1995-05-01
期刊：
影响因子：
12.4
通讯作者：
STEVENS, EAM
中科院分区：
文献类型：
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作者：
CADOT, P;LEJOLY, M;STEVENS, EAM
桦树(Betula Verrucosa)花粉提取液在不同pH条件下制备，pH值恒定监测，预设值在5.5-8.5之间。这些提取物的总蛋白含量与pH值呈正相关。考马斯亮蓝染色、等电聚焦、SDS-PAGE凝胶和免疫印迹分析显示出定性的差异：当pH改变时，一些蛋白质丢失，而另一些蛋白质出现。在pH 8.5时，约30%的桦树花粉敏感血清可检测到等电点为9、9.10和9.30的未知桦树花粉变应原。在pH接近中性，即6.5和7.5的条件下制备的桦树花粉提取物具有最大的蛋白质含量和不同的过敏原多样性。因此，萃取物的pH值对于分析萃取物中致敏成分的整体模式是必要的。
Extracts from birch (Betula verrucosa) pollen were prepared at different pH, with constant pH monitoring and adjustment to preset values in the range 5.5-8.5. The total protein content of these extracts was directly correlated with the pH. Coomassie brilliant blue-stained isoelectric focusing and SDS-PAGE gels and immunoblot analysis demonstrated qualitative differences: some proteins were lost while others appeared when pH was changed. At pH 8.5, formerly unknown birch pollen allergens were detected with pI 9, 9.10, and 9.30 by about 30% of birch pollen-sensitive sera. Birch pollen extracts prepared at a pH close to neutrality, namely, 6.5 and 7.5, showed the greatest protein and different allergen diversity. Thus, extraction pH values are necessary to analyze the whole pattern of allergenic components in an extract.