Effect of Ca+ on the photobactericidal efficacy of methylene blue and toluidine blue against gram-negative bacteria and the dye affinity for lipopolysaccharides.
Effect of Ca+ on the photobactericidal efficacy of methylene blue and toluidine blue against gram-negative bacteria and the dye affinity for lipopolysaccharides.
复制标题
Ca对亚甲蓝和甲苯胺蓝对革兰氏阴性菌的光杀菌效果以及对脂多糖的染料亲和力的影响。
DOI：
10.1002/lsm.20400
复制
发表时间：
2006
影响因子：
2.4
通讯作者：
Biel,MerrillA
中科院分区：
文献类型：
--
作者：
Usacheva,MarinaN;Teichert,MatthewC;Sievert,ChetE;Biel,MerrillA
背景与目的亚甲蓝（MB）和甲苯胺蓝（TB）与脂多糖（LPS）形成异染性复合物。TB的更大的光杀菌功效可以通过其对LPS的亲和力来解释。本研究旨在阐明使用Ca 2+作为细菌外膜上染料结合位点的竞争者的染料之间的光杀菌功效的差异。研究设计/材料和方法将具有革兰氏阴性菌的固定染料浓度溶液和增加浓度的CaCl 2暴露于红色激光。细菌的生存和分光光度法被用来描述的影响，Ca 2+对染料与细菌和LPS.ResultsMB介导的光杀伤抑制比TB更显着。CaCl 2抑制染料的光漂白作用，并抑制染料与LPS，特别是TB之间的异染反应。结论CaCl 2通过与LPS以及其他阴离子聚合物（包括外膜蛋白）结合抑制细菌的光杀伤作用。LPS主要参与TB介导的光杀伤，而外膜蛋白可能更多地参与MB介导的光杀伤。激光外科医学38：946-954，2006年。© 2006 Wiley利斯公司
Background and ObjectivesMethylene blue (MB) and toluidine blue (TB) form metachromatic complexes with lipopolysaccharides (LPS). The greater photobactericidal efficacy of TB may be explained by its affinity for LPS. This study aims to elucidate the difference in photobactericidal efficacies between the dyes using Ca2+as a competitor for dye‐binding sites on the bacterial outer membrane.Study Design/Materials and MethodsFixed dye concentration solutions with gram‐negative bacteria and increasing concentrations of CaCl2were exposed to red laser light. Bacterial survival and spectrophotometry were used to describe the effect of Ca2+on dye interaction with bacteria and LPS.ResultsMB‐mediated photokilling was inhibited more significantly than that of TB. CaCl2inhibited dye photobleaching and suppressed the metachromatic reaction between the dyes and LPS, in particular TB.ConclusionsCaCl2inhibits bacterial photokilling by binding with LPS, as well as other anionic polymers including outer membrane proteins. LPS is chiefly involved in TB‐mediated photokilling, whereas outer membrane proteins probably are more involved in MB‐mediated photokilling. Lasers Surg. Med. 38:946–954, 2006. © 2006 Wiley‐Liss, Inc.