Remineralization effectiveness of the PAMAM dendrimer with different terminal groups on artificial initial enamel caries in vitro.
Remineralization effectiveness of the PAMAM dendrimer with different terminal groups on artificial initial enamel caries in vitro.
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不同末端基团的 PAMAM 树枝状聚合物对体外人工牙釉质龋的再矿化效果。
DOI：
10.1016/j.dental.2019.11.015
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发表时间：
2020-02-01
期刊：
影响因子：
--
通讯作者：
Li, Jiyao
中科院分区：
文献类型：
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作者：
Fan, Menglin;Zhang, Min;Li, Jiyao
目的：脱矿-矿化平衡的破坏可能引发龋齿的发展，使牙釉质“自我愈合”具有挑战性。因此，需要外部帮助来恢复釉质损伤并停止破坏进展。本研究的目的是通过聚氨基胺（PAMAM）树枝状大分子定量研究模拟口腔环境中的釉质脱矿作用.方法：将牛牙釉质样品在脱矿溶液（pH 4.5，37°C，50 rpm/min）中振荡72 h，建立初始釉质龋损模型.然后将表面下脱矿的样品分为四组：用PAMAM-NH 2处理的釉质、用PAMAM-COOH处理的釉质、用PAMAM-OH处理的釉质和用去离子水处理的釉质。处理后的标本随后进行了12天的pH循环。结果：PAMAM树枝状大分子处理组牙釉质损伤深度较低，矿化损失较少，牙釉质垂直断面表面硬度恢复较好，矿化吸附量较多（P < 0. 05），牙釉质表面硬度恢复较好（P < 0. 05），牙釉质表面硬度恢复较好（P < 0. 05），牙釉质表面硬度恢复较好（P < 0. 05）。
OBJECTIVE: Disruption of the demineralization-remineralization balance could trigger the development of dental caries, making it challenging for enamel to "self-heal". Thus, extrinsic assistance is needed to restore enamel lesions and stop undermining progression. The aim of this study was to investigate enamel remineralization in a simulated oral environment via poly (amino amine) (PAMAM) dendrimers quantitatively.METHODS: Bovine enamel specimens were shaken in demineralization solution (pH 4.5, 37°C, 50rpm/min) for 72h to create initial enamel carious lesions. The subsurface-demineralized specimens were then divided into four groups: enamel treated with PAMAM-NH2, enamel treated with PAMAM-COOH, enamel treated with PAMAM-OH, and enamel treated with deionized water. The treated specimens underwent subsequent 12-day pH cycling. Enamel blocks were analyzed by transverse microradiography (TMR), surface microhardness testing and scanning electron microscopy (SEM) before and after demineralization and pH cycling.RESULTS: Groups treated with PAMAM dendrimers showed lower lesion depth and less mineral loss, attained more vertical-section surface microhardness recovery, and adsorbed more mineral deposits (p