Effect of melatonin on bone metabolism in ovariectomized rats
Effect of melatonin on bone metabolism in ovariectomized rats
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DOI：
10.1016/s0024-3205(01)01431-x
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发表时间：
2001-12-21
期刊：
影响因子：
6.1
通讯作者：
Cardinali, DP
中科院分区：
文献类型：
--
作者：
Ladizesky, MG;Cutrera, RA;Cardinali, DP
为了评估褪黑激素药理剂量对卵巢切除大鼠骨代谢的影响，在成年大鼠术后长达 60 天的时间内测量了尿脱氧吡啶啉（骨吸收的标志物）和钙排泄，钙、磷和骨碱性磷酸酶活性（骨形成的标志物）的循环水平，以及全身的骨矿物质密度（BMD）、矿物质含量（BMC）和骨面积（BA）。大鼠接受饮用水中的褪黑激素（25 杯/毫升水）或单独的饮用水。卵巢切除术后尿脱氧吡啶啉显着增加 51%（术后 30 天）和 47%（术后 60 天）。在接受褪黑激素治疗的大鼠中，未观察到卵巢切除后 30 天尿脱氧吡啶啉含量的增加。研究的 4 个实验组的尿钙浓度相似，每个检查时间间隔的循环钙浓度也相似。手术后十五天，与对照组相比，接受褪黑激素的卵巢切除大鼠的血清磷和骨碱性磷酸酶水平显着增加。手术后 60 天，切除卵巢的大鼠的 BMD、BMC 和 BA 显着下降，而褪黑激素并未改变这种效果。卵巢切除术后 30 天，血清雌二醇显着下降，到卵巢切除术后 60 天，血清雌二醇值接近检测限。结果支持这样的结论：药理学量的褪黑激素可以改变卵巢切除术后的骨重塑，并且该效果可能需要足够浓度的雌二醇。 (C) 2001 Elsevier Science Inc. 保留所有权利。
To assess the effect of pharmacological dose of melatonin on bone metabolism in ovariectomized rats, urinary deoxypyridinoline (a marker of bone resorption) and calcium excretion, circulating levels of calcium, phosphorus and bone alkaline phosphatase activity (a marker of bone formation), and bone mineral density (BMD), mineral content (BMC) and bone area (BA) of total body, were measured in adult rats for up to 60 days after surgery. Rats received melatonin in the drinking water (25 mug/ml water) or drinking water alone. Urinary deoxypyridinoline increased significantly after ovariectomy by 51% (30 days after surgery) and by 47% (60 days after surgery). The increase in urinary deoxypyridinoline found 30 days after ovariectomy was not observed in melatonin-treated rats. Urinary calcium concentration was similar in the 4 experimental groups studied, as was the circulating calcium concentration at every time interval examined. Fifteen days after surgery, a significant increase in serum phosphorus and bone alkaline phosphatase levels occurred in ovariectomized rats receiving melatonin as compared to their controls. Sixty days after surgery BMD, BMC and BA decreased significantly in ovariectomized rats, an effect not modified by melatonin. Serum estradiol decreased significantly by 30 days after ovariectomy to attain values close to the limit of detection of the assay by 60 days after ovariectomy. The results support the conclusion that a pharmacological amount of melatonin modifies bone remodeling after ovariectomy and that the effect may need adequate concentrations of estradiol. (C) 2001 Elsevier Science Inc. All rights reserved.