Borrelia burgdorferi sensu lato Infecting Ticks of the Ixodes ricinus Complex in Uruguay: First Report for the Southern Hemisphere
Borrelia burgdorferi sensu lato Infecting Ticks of the Ixodes ricinus Complex in Uruguay: First Report for the Southern Hemisphere
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DOI：
10.1089/vbz.2012.1102
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发表时间：
2013-03-01
影响因子：
2.1
通讯作者：
Labruna, Marcelo B.
中科院分区：
文献类型：
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作者：
Barbieri, Amalia M.;Venzal, Jose M.;Labruna, Marcelo B.
广义伯氏疏螺旋体（s.l.）组包括遗传上相关的螺旋体，大多数与属于北方半球的蓖麻硬蜱复合体的蜱种相关。本研究评估了副硬蜱（硬蜱属唯一代表性种）中的疏螺旋体感染。乌拉圭的蓖麻综合体。共137个I。从乌拉圭2个省的鹿、牛或植被中收集副蜱。通过PCR靶向疏螺旋体基因鞭毛蛋白（弗拉）直接测试这些蜱的一部分，而将另一部分蜱接种到Barbour-Stoenner-Kelly（BSK）-H培养基中以尝试分离疏螺旋体。总体而言，在9只雄性蜱和1只蜱若虫库中检测到疏螺旋体感染。这些蜱被发现由独特的弗拉单倍型感染，通过系统发育分析表明，这可能代表2个新的B。布格多费里公司基因种，1个与B相关。bissettii，另一个在遗传上最接近B。美国。这些结果通过PCR和DNA测序分析2个额外的疏螺旋体基因的部分，rrfA-rrlB基因间间隔区（IGS）和16 S rDNA（rrs）得到加强。通过暗视野显微镜对BSK培养物进行每周检查，未能通过100天的潜伏期证明活疏螺旋体。然而，通过fla-PCR在接种后长达90天的2个小瓶的培养基中检测到疏螺旋体DNA。据我们所知，这是B的首次报告。布格多费里公司感染南美洲的蜱虫
The Borrelia burgdorferi sensu lato (s.l.) group comprises genetically related spirochetes, mostly associated with tick species belonging to the Ixodes ricinus complex in the Northern Hemisphere. The present study evaluated borrelial infection in the tick Ixodes pararicinus, which is the only representative species of the I. ricinus complex in Uruguay. A total of 137 I. pararicinus ticks were collected from deer, cattle, or vegetation in 2 Uruguayan Departments. A part of these ticks was tested directly by PCR targeting the borrelial gene flagellin (fla), whereas another part of the ticks was inoculated into Barbour-Stoenner-Kelly (BSK)-H medium in an attempt to isolate Borrelia. Overall, Borrelia infection was detected in 9 males and 1 nymphal tick pool. These ticks were found to be infected by unique fla haplotypes, which were shown through phylogenetic analysis to represent possibly 2 new B. burgdorferi s.l. genospecies, 1 associated with B. bissettii, the other phylogenetically closest to B. americana. These results were reinforced by PCR and DNA sequencing analyses of portions of 2 additional borrelial genes, rrfA-rrlB intergenic spacer region (IGS) and 16S rDNA (rrs). Weekly examinations of BSK cultures by dark-field microscopy failed to demonstrate live Borrelia through a 100-day incubation period. However, Borrelia DNA was detected by fla-PCR in culture media from 2 vials up to 90 days after inoculation. To the best of our knowledge, this is the first report of B. burgdorferi s.l. infecting ticks in South America.