Structural abnormalities develop in the brain after ablation of the gene encoding nonmuscle myosin II-B heavy chain
Structural abnormalities develop in the brain after ablation of the gene encoding nonmuscle myosin II-B heavy chain
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DOI：
10.1002/cne.1125
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发表时间：
2001-04-23
影响因子：
2.5
通讯作者：
Hara, Y
中科院分区：
文献类型：
--
作者：
Tullio, AN;Bridgman, PC;Hara, Y
消融小鼠非肌肉肌球蛋白重链II-B(NMHC-B)可导致严重脑积水，并伴有侧脑室和第三脑室扩大。所有B*/B*小鼠在胚胎发育过程中或出生当天(PO)死亡。与正常小鼠相比，培养于胚胎第18天至P0天的B*/B*小鼠颈上神经节的神经元突起生长速度降低，其生长锥体具有明显的狭窄形态。E12.5、E13.5和E15小鼠大脑的连续切片发现了脑室神经上皮的发育缺陷。E12.5可见室壁内不同节段的神经上皮细胞和分化细胞的粘连表面被破坏，细胞迁移紊乱。这些异常导致在大脑和脊髓的不同区域形成玫瑰花环。在E13.5和E15，脑室表面的破坏和神经细胞向脑室的异常突起更加突出。到E18.5和P0时，由于第三脑室和脑导水管狭窄或闭塞，导致脑室壁细胞缺陷，导致梗阻性脑积水。这些缺陷可能是由神经上皮细胞的细胞黏附特性异常引起的，表明NMHC-B在哺乳动物大脑的早期和晚期发育过程中都是必不可少的。2001年出版，Wiley-Liss，Inc.(Dagger)。
Ablation of nonmuscle myosin heavy chain II-B (NMHC-B) in mice results in severe hydrocephalus with enlargement of the lateral and third ventricles. All B*/B* mice died either during embryonic development or on the day of birth (PO). Neurons cultured from superior cervical ganglia of B*/B* mice between embryonic day (E) 18 and P0 showed decreased rates of neurite outgrowth, and their growth cones had a distinctive narrow morphology compared with those from normal mice. Serial sections of E12.5, E13.5, and E15 mouse brains identified developmental defects in the ventricular neuroepithelium. On E12.5, disruption of the coherent ventricular surface and disordered cell migration of neuroepithelial and differentiated cells were seen at various points in the ventricular walls. These abnormalities resulted in the formation of rosettes in various regions of the brain and spinal cord. On E13.5 and E15, disruption of the ventricular surface and aberrant protrusions of neural cells into the ventricles became more prominent. By E18.5 and P0, the defects in cells lining the ventricular wall resulted in an obstructive hydrocephalus due to stenosis or occlusion of the third ventricle and cerebral aqueduct. These defects may be caused by abnormalities in the cell adhesive properties of neuroepithelial cells and suggest that NMHC-B is essential for both early and late developmental processes in the mammalian brain. Published 2001 Wiley-Liss, inc.(dagger).