Mesenchymal progenitor cells in human umbilical cord blood
Mesenchymal progenitor cells in human umbilical cord blood
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DOI：
10.1046/j.1365-2141.2000.01986.x
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发表时间：
2000-04-01
影响因子：
6.5
通讯作者：
Minguell, JJ
中科院分区：
文献类型：
--
作者：
Erices, A;Conget, P;Minguell, JJ
造血由两种主要的细胞成分维持，即造血细胞（HSC）和间充质祖细胞（MPC）。MPCs是多能性的，是骨髓基质、骨、软骨、肌肉和结缔组织的前体。尽管脐带血（UCB）中存在HSC是众所周知的，但MPCs的存在尚未得到充分评价。在这项研究中，我们检查了UCB收获物在培养细胞中产生具有MPC特征的细胞的能力。结果表明，脐血来源的单核细胞，当设置在文化中，产生贴壁细胞，表现出破骨细胞或间充质样表型。具有破骨细胞表型的细胞是多核的，表达TRAP活性和抗原CD 45和CD 51/CD 61。进而，具有间充质表型的细胞显示成纤维细胞样形态，并表达几种MPC相关抗原（SH 2、SH 3、SH 4、ASMA、MAB 1470、CD 13、CD 29和CD 49 e）。我们的研究结果表明，早产儿，与长期相比，脐带血中更丰富的间充质祖细胞，类似于造血祖细胞。
Haemopoiesis is sustained by two main cellular components, the haematopoietic cells (HSCs) and the mesenchymal progenitor cells (MPCs). MPCs are multipotent and are the precursors for marrow stroma, bone, cartilage, muscle and connective tissues, Although the presence of HSCs in umbilical cord blood (UCB) is well known, that of MPCs has been not fully evaluated. In this study, we examined the ability of UCB harvests to generate in culture cells with characteristics of MPCs. Results showed that UCB-derived mononuclear cells, when set in culture, gave rise to adherent cells, which exhibited either an osteoclast- or a mesenchymal-like phenotype. Cells with the osteoclast phenotype were multinucleated, expressed TRAP activity and antigens CD45 and CD51/CD61. In turn, cells with the mesenchymal phenotype displayed a fibroblast-like morphology and expressed several MPC-related antigens (SH2, SH3, SH4, ASMA, MAB 1470, CD13, CD29 and CD49e). Our results suggest that preterm, as compared with term, cord blood is richer in mesenchymal progenitors, similar to haematopoietic progenitors.