Genome-wide miRNA screening reveals miR-310 family members negatively regulate the immune response in Drosophila melanogaster via co-targeting Drosomycin
Genome-wide miRNA screening reveals miR-310 family members negatively regulate the immune response in Drosophila melanogaster via co-targeting Drosomycin
复制标题
全基因组 miRNA 筛选揭示 miR-310 家族成员通过共靶向 Drosomycin 负向调节果蝇的免疫反应
DOI：
10.1016/j.dci.2016.11.014
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发表时间：
2017-03-01
影响因子：
2.9
通讯作者：
Ma, Fei
中科院分区：
文献类型：
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作者：
Li, Yao;Li, Shengjie;Ma, Fei
虽然Toll信号介导的先天免疫已经在果蝇中得到了广泛的研究，但miRNA在调节Toll介导的免疫应答中的作用仍然很大程度上未知。在这项研究中，在革兰氏阳性细菌挑战后，我们通过全基因组miRNA筛选鉴定了93种差异表达的miRNA。这些miRNAs被认为与免疫应答相关（IRR）。通过对41个UAS-miRNA株系进行遗传筛选，从93个IRR miRNAs中筛选出60个miRNAs，证实8个miRNAs参与了革兰氏阳性菌感染后Toll介导的免疫应答。有趣的是，这八种miRNA中的四种，miR-310，miR-311，miR-312和miR-313，是成簇的miRNA，属于miR-310家族。这些miR-310家族成员显示出靶向和调节由Toll信号传导产生的抗微生物肽Drosomycin的表达。综上所述，我们的研究暗示了miRNA在革兰氏阳性细菌感染后果蝇Toll介导的先天免疫应答中的重要调节作用。(C)2016爱思唯尔有限公司版权所有
Although innate immunity mediated by Toll signaling has been extensively studied in Drosophila melanogaster, the role of miRNAs in regulating the Toll-mediated immune response remains largely unknown. In this study, following Gram-positive bacterial challenge, we identified 93 differentially expressed miRNAs via genome-wide miRNA screening. These miRNAs were regarded as immune response related (IRR). Eight miRNAs were confirmed to be involved in the Toll-mediated immune response upon Gram-positive bacterial infection through genetic screening of 41 UAS-miRNA lines covering 60 miRNAs of the 93 IRR miRNAs. Interestingly, four out of these eight miRNAs, miR-310, miR-311, miR-312 and miR-313, are clustered miRNAs and belong to the miR-310 family. These miR-310 family members were shown to target and regulate the expression of Drosomycin, an antimicrobial peptide produced by Toll signaling. Taken together, our study implies important regulatory roles of miRNAs in the Toll-mediated innate immune response of Drosophila upon Gram-positive bacterial infection. (C) 2016 Elsevier Ltd. All rights reserved.