EVIDENCE THAT RESERVE CELLS ARE A SOURCE OF REGENERATED ADULT NEWT MUSCLE INVITRO
EVIDENCE THAT RESERVE CELLS ARE A SOURCE OF REGENERATED ADULT NEWT MUSCLE INVITRO
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DOI：
10.1038/321607a0
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发表时间：
1986-06-05
期刊：
影响因子：
64.8
通讯作者：
HINTERBERGER, TJ
中科院分区：
文献类型：
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作者：
CAMERON, JA;HILGERS, AR;HINTERBERGER, TJ
成年、变态的尾鱼两栖动物的骨骼肌在没有卫星细胞的情况下会再生1，成肌储备细胞存在于尾鱼幼虫和其他脊椎动物的肌肉纤维外层之下2-9。几种尾目动物成年期的肌肉(绿色眼镜蛇1，10，斑点无毛虫10，普通金鱼，鸡尾鱼11，小眼鱼12和墨西哥带鱼13)有一种独特的细胞类型被包裹在自己的外层中。几项研究表明，这些细胞是幼虫卫星细胞，在变态10-12期间完全被一段外板包围。尽管Popiela10将这些细胞称为“周细胞”，但他认为它们可能是一种肌源性储备细胞群。切尔卡索娃是第一个认识到这些细胞有别于血管周细胞的人，他将它们命名为卫星后细胞，并提出它们可能是肌源性细胞。在这里，我们提出了成年后卫星细胞的证据。绿僵菌在体外产生再生的肌管。肌管可以用纯化的骨骼肌肌球蛋白14的单抗MF20染色，也可以用22/18的单抗染色，22/18的单抗与新生肢体胚基细胞15中存在的抗原发生反应。成体Newt肌肉中的成肌性卫星后细胞的存在为成肌细胞的再生提供了另一种假说，即成肌细胞是通过成熟肌纤维的去分化而产生的。
Skeletal muscle of adult, metamorphosed urodele amphibians regenerates despite the absence of satellite cells1, the myogenic reserve cells present beneath the external lamina of muscle fibres in larval urodeles and in other vertebrates2–9. Adult-stage muscles of several urodeles examined (Notophthalmus viridescens1,10,Ambystoma maculatum10,Triturus vulgaris,Triturus cristatus11,Hynobius tokyoensis12andAmbystoma mexicanum13) have instead a unique cell type enveloped in its own external lamina. Several studies suggest that these cells are larval satellite cells that have become completely surrounded by a segment of external lamina during metamorphosis10–12. Although Popiela10termed these cells ‘pericytes’, he suggested that they might function as a myogenic reserve population. Cherkasova, who was the first to recognize these cells as distinct from vascular pericytes, termed them post-satellite cells and also proposed that they might be myogenic11. Here, we present evidence that post-satellite cells in adultN. viridescensgive rise to regenerated myotubesin vitro. The myotubes could be stained with MF20, a monoclonal antibody to purified skeletal muscle myosin14, and also with 22/18, a monoclonal antibody that reacts with an antigen present in newt-limb blastema myogenic cells15. The presence of myogenic post-satellite cells in adult newt muscle provides an alternative to the hypothesis that myoblasts in regenerating adult urodele limbs arise through dedifferentiation of mature myofibres15–17.