Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor
Bacterial structural genomics target enabled by a recently discovered potent fungal acetyl-CoA synthetase inhibitor
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最近发现的有效真菌乙酰辅酶A合成酶抑制剂实现了细菌结构基因组学目标
DOI：
10.1107/s2053230x23003801
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Abendroth, Jan
中科院分区：
文献类型：
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作者：
DeBouver, Nicholas D.;Bolejack, Madison J.;Esan, Taiwo E.;Krysan, Damian J.;Hagen, Timothy J.;Abendroth, Jan
化合物乙基-腺苷单磷酸酯（乙基-AMP）已显示出有效地抑制乙酰辅酶A合成酶（ACS）并在各种情况下促进真菌ACS酶的结晶。在这项研究中，将乙基-AMP添加到嗜肺军团菌的细菌ACS中，导致确定了这种以前难以捉摸的结构基因组学靶点的共晶体结构。乙基-AMP在抑制ACS酶和促进结晶方面的双重功能确立了其作为推进这类蛋白质结构研究的宝贵资源的重要性。
The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.