MURINE TYPHUS - UPDATED ROLES OF MULTIPLE URBAN COMPONENTS AND A 2ND TYPHUSLIKE RICKETTSIA
MURINE TYPHUS - UPDATED ROLES OF MULTIPLE URBAN COMPONENTS AND A 2ND TYPHUSLIKE RICKETTSIA
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DOI：
10.1093/jmedent/31.5.681
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发表时间：
1994-09-01
影响因子：
2.1
通讯作者：
AZAD, AF
中科院分区：
文献类型：
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作者：
SCHRIEFER, ME;SACCI, JB;AZAD, AF
利用血清学和聚合酶链式反应(PCR)对德克萨斯州南部的野外样本进行的研究表明，存在伤寒立克次体和ELB病原体感染的猫蚤，以及首次观察到感染ELB的脊椎动物(负鼠)。ELB是一种最近描述的斑疹伤寒样立克次体，目前可用的血清学试剂与伤寒或普罗瓦泽克立克次体没有区别。用限制性内切酶对399只蚤类进行限制性内切酶检测，ELB菌感染率为3.8%，伤寒杆菌感染率为0.8%。被测试的九只负鼠中有三只(Didelphis Virginiana)被证明携带ELB病毒感染。在调查样本中未检出鼠伤寒杆菌感染鼠褐家鼠或印鼠客蚤Rothschild。这一鼠斑疹伤寒疫源地的持久性似乎更好地解释了受感染的猫蚤、负鼠和其他与人类种群密切相关的非老鼠宿主的存在。疑似病媒和宿主中斑疹伤寒的流行表明ELB试剂参与了小鼠斑疹伤寒的生物学研究。
Studies using serologic and polymerase chain reaction-(PCR) facilitated analysis of field samples from southern Texas indicate the presence of Rickettsia typhi and ELB agent infected cat fleas, Ctenocephalides felis (Bouche), and the first observation of ELB infected vertebrates (opossums). The ELB agent is a recently described typhus-like rickettsia that is not distinguished from R. typhi or R. prowazekii by currently available serologic reagents. Restriction digests of PCR products from 399 fleas revealed an ELB agent infection rate of 3.8% and a R. typhi infection rate of 0.8%. Three of nine tested opossums (Didelphis virginiana) were shown to harbor ELB agent infections. No R. typhi infected rats, Rattus norvegicus, or rat-fleas, Xenopsylla cheopis Rothschild, were detected among surveyed samples. The persistence of this murine typhus disease focus appears to be better accounted for by the presence of infected cat fleas, opossums, and other non-rat hosts found in close association with human populations. Involvement of the ELB agent in the biology of murine typhus is suggested by its prevalence among suspected vectors and reservoir hosts.