Immunohistochemical staining of radixin and moesin in prostatic adenocarcinoma.
Immunohistochemical staining of radixin and moesin in prostatic adenocarcinoma.
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DOI：
10.1186/1472-6890-11-1
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发表时间：
2011-01-14
影响因子：
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通讯作者：
Parwani AV
中科院分区：
文献类型：
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作者：
Bartholow TL;Chandran UR;Becich MJ;Parwani AV
蛋白质4.1超家族的一些成员被认为参与细胞增殖和生长，或参与这些过程的调节。虽然已经在许多肿瘤类型中研究了该家族的两个成员Radixin和Moesin的表达水平，但据我们所知，尚未在前列腺癌中研究它们。对组织微阵列进行放射状蛋白或膜突蛋白的电化学染色，随后使用单向ANOVA或非参数等效物对染色强度进行定量和统计学分析，随后使用Student-Newman-Keuls检验进行多重比较。在微阵列中分析了11例正常供体前列腺（NDP）、14例良性前列腺增生（BPH）、23例高度前列腺上皮内瘤变（HGPIN）、88例前列腺腺癌（PCa）和25例腺癌旁正常组织（NAC）。NDP、BPH和HGPIN具有比PCa和NAC更高的根蛋白绝对染色评分，但在仅HGPIN和PCa（p = < 0.001）与HGPIN和NAC（p = 0.001）之间观察到显著差异。在Moesin染色标本中，PCa、NAC、HGPIN和BPH的染色评分均高于NDP，但差异不显著。与第2阶段相比，第4阶段膜突蛋白染色的PCa具有显著降低的染色强度（p = 0.003）。据我们所知，这些研究代表了前列腺腺癌中的根蛋白和膜突蛋白的表达谱的第一个报告。目前的研究表明，在HGPIN和PCa以及HGPIN和NAC之间观察到的根蛋白表达存在统计学显著差异。不同组织类型的膜突蛋白谱差异无统计学意义。这些标记物的其他更大的研究可能进一步阐明其在前列腺肿瘤进展中的潜在作用。
Some members of the Protein 4.1 superfamily are believed to be involved in cell proliferation and growth, or in the regulation of these processes. While the expression levels of two members of this family, radixin and moesin, have been studied in many tumor types, to our knowledge they have not been investigated in prostate cancer. Tissue microarrays were immunohistochemically stained for either radixin or moesin, with the staining intensities subsequently quantified and statistically analyzed using One-Way ANOVA or nonparametric equivalent with subsequent Student-Newman-Keuls tests for multiple comparisons. There were 11 cases of normal donor prostates (NDP), 14 cases of benign prostatic hyperplasia (BPH), 23 cases of high-grade prostatic intraepithelial neoplasia (HGPIN), 88 cases of prostatic adenocarcinoma (PCa), and 25 cases of normal tissue adjacent to adenocarcinoma (NAC) analyzed in the microarrays. NDP, BPH, and HGPIN had higher absolute staining scores for radixin than PCa and NAC, but with a significant difference observed between only HGPIN and PCa (p = < 0.001) and HGPIN and NAC (p = 0.001). In the moesin-stained specimens, PCa, NAC, HGPIN, and BPH all received absolute higher staining scores than NDP, but the differences were not significant. Stage 4 moesin-stained PCa had a significantly reduced staining intensity compared to Stage 2 (p = 0.003). To our knowledge, these studies represent the first reports on the expression profiles of radixin and moesin in prostatic adenocarcinoma. The current study has shown that there were statistically significant differences observed between HGPIN and PCa and HGPIN and NAC in terms of radixin expression. The differences in the moesin profiles by tissue type were not statistically significant. Additional larger studies with these markers may further elucidate their potential roles in prostatic neoplasia progression.