Expression of endogenous beta retroviruses and Hyal-2 mRNA in immune organs of fetuses and lambs
Expression of endogenous beta retroviruses and Hyal-2 mRNA in immune organs of fetuses and lambs
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DOI：
10.1007/s12250-012-3222-4
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发表时间：
2012-04
影响因子：
5.5
通讯作者：
J. Qi;Xiao-Li Wu;Shu-Ying Liu;G. Cao
中科院分区：
文献类型：
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作者：
J. Qi;Xiao-Li Wu;Shu-Ying Liu;G. Cao
内源性β逆转录病毒（enJSRV）与绵羊肺腺癌（OPA）的病原羊肺腺瘤病毒（exJSRV）高度同源。本研究的目的是阐明在JSRV感染的绵羊血清中检测不到的enJSRV的功能及其相应抗体的免疫学机制。采用Real-Time RT-PCR和原位杂交技术检测了enJSRV囊膜蛋白和Hyal-2 mRNA在绵羊胎羊和羔羊免疫器官和肺组织中的表达，原位杂交结果表明，enJSRV囊膜蛋白和Hyal-2 mRNA在胸腺、脾脏、肠系膜淋巴结和肺组织中均有不同时间的表达，而阴性对照组均未检测到阳性信号。Real-Time RT-PCR分析结果表明，在130日龄胎羊和3日龄新生羔羊中，与免疫系统相关的器官中enJSRV mRNA的表达水平明显高于肺，尤其是胸腺和脾脏，而Hyal-2 mRNA的表达水平在所有组织中差异不显著。这些结果从免疫学的角度提供了证据，以了解为什么在JSRV感染的绵羊中检测不到针对exJSRV的循环抗体，并将有助于阐明JSRV感染的绵羊的发病机制。
Endogenous beta retroviruses (enJSRV) are highly homologous with Jaagsiekte sheep retrovirus (exJSRV), this exogenous retrovirus is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). The aim of this study was to clarify the function of enJSRV and the immunological mechanisms of its corresponding antibody, that is undetectable in JSRV-infected ovine serum. The expression of enJSRV envelope protein and Hyal-2 mRNA in immune organs and lungs of ovine fetuses and lambs were analyzed by Real-Time reverse transcription PCR andIn SituHybridization using specific probes.In SituHybridization results indicated that the enJSRV envelope protein and Hyal-2 mRNA were expressed in thymus, spleen, mesenteric lymph nodes and lungs at different times, while no positive signals were detected in the negative controls. On the other hand, results from Real-Time reverse transcription PCR analysis showed that in 130d fetuses and 3d newborn lambs the enJSRV mRNA levels were much higher in organs associated with the immune system than that in lungs, especially in the thymus and spleen, but levels of Hyal-2 mRNA expression was not significantly different in all collected tissue. These results provided evidence from an immunology point of view to understand why the circulating antibodies against exJSRV are undetectable in JSRV-infected ovine, and will help to unravel the pathogenesis of JSRV-infected ovine.