Cytological properties of stromal cells derived from giant cell tumor of bone (GCTSC) which can induce osteoclast formation of human blood monocytes without cell to cell contact
Cytological properties of stromal cells derived from giant cell tumor of bone (GCTSC) which can induce osteoclast formation of human blood monocytes without cell to cell contact
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DOI：
10.1016/j.orthres.2005.01.004
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发表时间：
2005-09-01
影响因子：
2.8
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Nishimura, M;Yuasa, K;Ito, Y
当人血单核细胞与来源于人骨巨细胞瘤（GCTSC）的基质细胞共培养时，在单核细胞和GCTSC之间插入Millipore滤器（0.4 μ m），诱导单核细胞的多核巨细胞形成。多核巨细胞具有破骨细胞样细胞的特征，表明表达RANK、RANKL/ODF/OPGL和TACE mRNA的GCTSC分泌可溶性破骨细胞诱导因子。此外，OCIF/OPG抑制GCTSC诱导的破骨细胞生成，表明RANK-RANKL系统参与GCTSC诱导的破骨细胞生成，并且可溶性形式的ODF/RANKL从单核细胞诱导破骨细胞。GCTSC表达细胞因子mRNA，如M-CSF、GM-CSF、IL-3、IL-4、fL-6和IFN-γ mRNA。在所有培养基中均未检测到IL-1 r α、IL-1 β、IL-2、IL-4、IL-10、IL-18、TNF-α、G-CSF和IFN-γ。所有GCTSC的培养液中均可检测到大量IL-6。在两个GCTSC和两个骨肉瘤衍生细胞的培养基中发现IL-8。在所有培养基中均检测到M-CSF。GCTSC表达CaSR，细胞外Ca ~（2+）或CaSR激动剂新霉素刺激GCTSC可增加RANKL的表达。部分GCTSC表达碱性磷酸酶、骨钙素和Cbfa 1，提示GCTSC与成骨细胞系密切相关。(c)2005骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
When human blood monocytes were cocultured with stromal cells derived from human giant cell tumor of bone (GCTSC) and a Millipore filter (0.4 mu m) was interposed between monocytes and GCTSC, multinucleated giant cell formation of monocytes was induced. The multinucleated giant cells have characters as osteoclast-like cells, indicating that a soluble osteoclast-inducing factor(s) is secreted from GCTSC expressing RANK, RANKL/ODF/OPGL and TACE mRNA. Furthermore, OCIF/OPG inhibited GCTSC-induced osteoclastogenesis, showing that the RANK-RANKL system is involved in GCTSC-induced osteoclastogenesis and that soluble form of ODF/RANKL induces osteoclasts from inonocytes. GCTSC expressed the cytokine mRNAs such as M-CSF, GM-CSF, IL-3, IL-4, fL-6, and IFN-gamma mRNAs. None of IL-1r alpha, IL-1 beta, IL-2, IL-4, IL-10, IL-18, TNF-alpha, G-CSF and IFN-gamma could be detected in all culture media. A significant amount of IL-6 could be detected in the culture media of all GCTSC. IL-8 was found in the culture media of two GCTSC and two osteosarcoma-derived cells. M-CSF was detected in all culture media. GCTSC express CaSR, and stimulation of GCTSC with either extracellular Ca2+ or neomycin, agonist of CaSR, augumented the expression of RANKL. Some lines of GCTSC expressed alkaline phosphatase, osteocalcin and Cbfa1, suggesting that GCTSC are intimately related to osteoblastic lineage. (c) 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.