Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry
Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry
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DOI：
10.1002/rcm.4696
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发表时间：
2010-09-01
影响因子：
2
通讯作者：
Pistocchi, Rossella
中科院分区：
文献类型：
--
作者：
Ciminiello, Patrizia;Dell'Aversano, Carmela;Pistocchi, Rossella
在过去的几十年里，意大利的海岸线一直受到底栖甲藻卵形Ostreopsis ovata反复出现的困扰。这种藻类因其产生类似鬼臼毒素的化合物而造成严重的卫生紧急情况和经济损失。以往的研究已经证实，卵毒素-a(OVTX-a)是藻类毒素谱中的主要毒素，同时还有少量推定的孢子虫毒素(PLTX)。在我们正在进行的关于卵圆线虫毒素的研究中，我们在此报告了利用高分辨率(HR)液相色谱/质谱仪(LC/MS)和串联质谱仪(MS(2))对卵圆线虫培养物进行的深入调查。特别是，证实了推测的PLTX和OVTX-a的存在，并强调了在提取物中存在四个新的类似孢子虫毒素的化合物：OVTX-b、-c、-d和-e。新的卵毒素已被指定为元素式，并已获得关于其结构特征的信息。对卵母细胞培养物的定量研究表明，所有新的卵毒素约占总毒素含量的46%，因此，在进行基于LC/MS的浮游生物或受污染海产品的监测时，必须考虑它们的存在。版权所有(C)2010 John Wiley&Sons，Ltd.
Over the past decades, Italian coastlines have been plagued by recurring presence of the benthic dinoflagellate Ostreopsis ovata. Such an alga has caused severe sanitary emergencies and economic losses due to its production of palytoxin-like compounds. Previous studies have confirmed the presence of ovatoxin-a (OVTX-a) as the major toxin of the algal toxin profile together with small amounts of putative palytoxin (PLTX). In our ongoing research on O. ovata toxins we report herein on in-depth investigation of an O. ovata culture carried out by high-resolution (HR) liquid chromatography/mass spectrometry (LC/MS) and tandem mass spectrometry (MS(2)). Particularly, the presence of putative PLTX and OVTX-a was confirmed and the occurrence in the extract of four new palytoxin-like compounds, OVTX-b, -c, -d, and -e, was highlighted. Elemental formulae have been assigned to the new ovatoxins and information has been gained about their structural features. A quantitative study of the O. ovata culture extract indicated that the whole of the new ovatoxins represents about 46% of the total toxin content and, thus, their presence has to be taken into account when LC/MS-based monitoring programs of either plankton or contaminated seafood are carried out. Copyright (c) 2010 John Wiley & Sons, Ltd.