The ecological roles of Bacillus thuringiensis within phyllosphere environments.
The ecological roles of Bacillus thuringiensis within phyllosphere environments.
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DOI：
10.1016/j.chemosphere.2014.01.050
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发表时间：
2014-08
期刊：
影响因子：
8.8
通讯作者：
Xiaoxian Wang;Ya-rong Xue;Mei-hua Han;Y. Bu;Changhong Liu
中科院分区：
文献类型：
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作者：
Xiaoxian Wang;Ya-rong Xue;Mei-hua Han;Y. Bu;Changhong Liu
苏云金芽孢杆菌（Bacillus thuringiensis, Bt）是目前应用最广泛的植物害虫生物防治剂之一，但其对植物叶片微生物种群和群落的影响尚不清楚。利用培养依赖法观察到，Bt在叶层内的动态随Bt喷施剂量和植物种类的不同而变化，但接种15 d后Bt的种群规模稳定在1000 cfu g−1左右。通过比较3种植物叶层内的细菌丰度和群落结构，通过培养依赖试验证实，分别为1.5 × 107和1.5 × 109cfu mL - 1的Bt对叶片表面天然细菌种群大小没有显著影响。然而，基于培养非依赖性变性梯度凝胶电泳（DGGE）、Shannon-Wiener指数（H’）和UPGMA分析，Bt对苋菜和棉花层内细菌群落有显著影响，而对水稻层内细菌群落没有显著影响。这些结果表明，Bt对层圈内微生物多样性表现出不同的行为和生态作用，其环境安全性有待进一步关注和评价。
Bacillus thuringiensis(Bt) is one of the most used bio-control agents to control plant insects, but little is known about its effect on the microbial population and communities on plant leaves. With the culture dependent method, it has been observed that the dynamics of Bt within the phyllosphere varied dependent on both the doses of Bt sprayed on the leaves and the plant species, however, Bt’s population size kept stable at about 1000 cfu g−1after 15 d since inoculation. By comparing the bacterial abundances and community structures within the phyllosphere of three plant species, we confirmed that Bt at the doses of 1.5 × 107and 1.5 × 109cfu mL−1respectively did not significantly influence the natural bacterial population size on the leaf surfaces based on culture dependent assay. However, based on culture independent denaturing gradient gel electrophoresis (DGGE), Shannon–Wiener index (H′) and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis, Bt has a significant influence on the bacterial communities within the phyllosphere of amaranth and cotton, but not rice. These results indicate that Bt exhibits different behaviors and ecological roles on the microbial diversity within the phyllosphere, and its environmental safety has to be concerned and evaluated in the future.