Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp nov and Clostridium saccharobutylicum sp nov.
Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp nov and Clostridium saccharobutylicum sp nov.
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DOI：
10.1099/00207713-51-6-2095
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发表时间：
2001-11-01
影响因子：
2.8
通讯作者：
Jones, DT
中科院分区：
文献类型：
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作者：
Keis, S;Shaheen, R;Jones, DT
在16S rRNA基因测序和DNA-DNA再关联的基础上，将工业溶剂产梭菌划分为4种。本研究比较了乙酰丁酸梭菌（Clostridium acetobutylicum）、贝氏梭菌（Clostridium beijerinckii）、‘Clostridium saccharoperbutylacetonicum’以及以菌株NCP 262(T)和NRRL B643为代表的未命名梭菌的表型特征。此外，通过生物分型、DNA指纹图谱和16S rRNA基因测序对另外40株产溶剂梭菌进行了分类。其中包括14株贝氏梭菌，2株目前被命名为“卡内波梭菌”和“丁醇梭菌”，以及24株用于商业丙酮-丁醇发酵的生产菌株。所有菌株均被正确分类。“C. kaneboi”和“C, butanologenum”菌株需要分别重新分类为C: acetobutylicum和C. beijerinckii。商业生产菌株可能属于C. beijerinckii，也可能属于未命名的Clostridium sp.。为了进行4个物种的比较表型研究，从每个物种的dna指纹亚群中选择有代表性的菌株。分析了这些菌株利用不同碳水化合物、水解明胶或aesculin和产生吲哚的能力，并测试了过氧化氢酶和脲酶的存在。在这些结果的基础上，发现了一些表型性状对四个物种之间的区分有用。对C. acetobutylicum和C. beijerinckii的描述进行了修订。建议将这两个新种分别命名为Clostridium saccharoperbutylacetonicum sp. nov.[类型菌株=N1-4 (HMT) = ATCC 27021(T)]和Clostridium saccharobutylicum sp. nov.(类型菌株= DSM 13864（T) = ATCC BAA-117(T)）。
On the basis of 16S rRNA gene sequencing and DNA-DNA reassociation, industrial solvent-producing clostridia have been assigned to four species. In this study, the phenotypic characteristics of Clostridium acetobutylicum, Clostridium beijerinckii, 'Clostridium saccharoperbutylacetonicum', and an unnamed Clostridium sp. represented by the strains NCP 262(T) and NRRL B643 are compared. In addition, a further 40 strains of solvent-producing clostridia have been classified by biotyping, DNA fingerprinting and 16S rRNA gene sequencing. These included 14 C. beijerinckii strains, two strains currently designated as 'Clostridium kaneboi' and 'Clostridium butanologenum', and 24 production strains used in the commercial acetone-butanol fermentation. All of the C. beijerinckii strains were confirmed to have been classified correctly. The 'C. kaneboi' and 'C, butanologenum' strains require reclassification as C: acetobutylicum and C. beijerinckii, respectively. The commercial production strains were found to belong either to C. beijerinckii or to the unnamed Clostridium sp. For the comparative phenotypic studies of the four species, representative strains were selected from each of the DNA-fingerprint subgroups within each species. These strains were analysed for their ability to utilize different carbohydrates, hydrolyse gelatin or aesculin, and produce indole, and were tested for the presence of catalase and urease. On the basis of these results, several phenotypic traits were found to be useful for differentiating between the four species. The descriptions of C. acetobutylicum and C, beijerinckii have been emended. The names Clostridium saccharoperbutylacetonicum sp. nov. [type strain =N1-4 (HMT) = ATCC 27021(T)] and Clostridium saccharobutylicum sp. nov. (type strain = DSM 13864(T) = ATCC BAA-117(T)) are proposed for the two new species.