Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing.
Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing.
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用于伤口愈合的细胞接种、富含胶原蛋白的水凝胶的分析。
DOI：
10.1097/gox.0000000000003049
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Fox,PaigeM
中科院分区：
文献类型：
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作者：
SoteloLeon,Daniel;Williams,Tokoya;Wang,Zhen;Leyden,Jacinta;Franklin,Austin;Kaizawa,Yukitoshi;Chang,James;Fox,PaigeM
背景：我们实验室此前开发了一种新型富含胶原蛋白的水凝胶（cHG），可显着提高糖尿病大鼠伤口愈合的速度。方法：在本研究中，我们检测了新型cHG中成纤维细胞、内皮细胞和脂肪源性干细胞的体外存活和迁移情况。此外，我们测试了脂肪干细胞接种的 cHG 在体内支持细胞存活和加速愈合的能力。结果：在体外，cHG 内的细胞存活率保留了 25 天。我们无法检测细胞迁移入、移出或通过 cHG。在体内模型中，在糖尿病大鼠伤口的 cHG 内接种的干细胞的生物发光直到第 10 天才被检测到。从第 2 天到第 16 天，cHG 加脂肪干细胞的伤口闭合率高于对照组，并且在第 6、8 和 12 天显着（P < 0.05）。第 16 天的组织学也观察到了这种显着差异（P≤0.05）。结论：我们得出结论，cHG 是将脂肪干细胞、内皮细胞和成纤维细胞递送至伤口的良好候选者。未来的研究将确定 cHG 中不同细胞系的组合是否能进一步促进伤口愈合。
Background:Our laboratory has previously developed a novel collagen-rich hydrogel (cHG), which significantly increases the speed of wound healing in diabetic rats.Methods:In this study, we examine the in vitro survival and migration of fibroblasts, endothelial cells, and adipose-derived stem cells in a novel cHG. Furthermore, we test the ability of adipose-derived stem cell–seeded cHG to support cell survival and accelerate healing in vivo.Results:In vitro, cell survival within the cHG was retained for 25 days. We were unable to detect cellular migration into, out of, or through cHG. In the in vivo model, bioluminescence of stem cells seeded within the cHG in diabetic rat wounds was detected until day 10. Rate of wound closure was higher for cHG plus adipose-derived stem cells versus control from day 2 until day 16 and significant on days 6, 8, and 12 (P< 0.05). This significant difference was also observed on day 16 by histology (P≤ 0.05).Conclusions:We conclude that cHG is a good candidate for delivering adipose-derived stem cells, endothelial cells, and fibroblasts to wounds. Future studies will determine whether the delivery of combinations of different cell lines in cHG further enhances wound healing.