Human monocyte chemotactic proteins-2 and -3: Structural and functional comparison with MCP-1
Human monocyte chemotactic proteins-2 and -3: Structural and functional comparison with MCP-1
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DOI：
10.1002/jlb.59.1.67
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发表时间：
1996-01-01
影响因子：
5.5
通讯作者：
VanDamme, J
中科院分区：
文献类型：
--
作者：
Proost, P;Wuyts, A;VanDamme, J
在结构上，单核细胞趋化蛋白MCP-1、MCP-2和MCP-3形成C-C或β-趋化因子的亚家族。与其他趋化因子一样，MCP由多种细胞在细胞因子刺激下产生（白细胞介素-1、肿瘤坏死因子-α、干扰素-γ）、细菌和病毒产物或有丝分裂原，MCP-1水平在感染和炎症期间增强，其特征在于白细胞浸润。在体外，MCP对不同范围的靶细胞具有趋化性，并根据细胞类型和测试的趋化因子显示不同的特异性生物活性，MCP-3具有最广泛的范围，因为它激活单核细胞、树突细胞、淋巴细胞、自然杀伤细胞、嗜酸性粒细胞、嗜碱性粒细胞和嗜中性粒细胞。对所有三种MCP最敏感的细胞是淋巴细胞和单核细胞，MCP-1是有效的嗜碱性粒细胞激活剂，但不吸引嗜酸性粒细胞，而在较高浓度下，MCP-2也刺激嗜酸性粒细胞和嗜碱性粒细胞两者，单核细胞上MCP的信号传导涉及至少两种G蛋白连接的C-C趋化因子受体：C-C CKR-1结合MCP-3，C-C CKR-2结合MCP-1和MCP-S，但不结合MCP-2。受体结合导致除MCP-2外的所有趋化因子的[Ca 2 +]（i）增强。
Structurally, the monocyte chemotactic proteins MCP-1, -2, and -3 form a subfamily of the C-C or beta-chemokines. Like other chemokines, MCPs are produced by a variety of cells on stimulation with cytokines (interleukin-1, tumor necrosis factor-alpha, interferon-gamma), bacterial and viral products or mitogens, MCP-1 levels are enhanced during infection and inflammation, which are characterized by leukocyte infiltration, In vitro, MCPs are chemotactic for a distinct spectrum of target cells and show different specific biological activities depending on the cell type and the chemokine tested, MCP-3 has the broadest range in that it activates monocytes, dendritic cells, lymphocytes, natural killer cells, eosinophils, basophils, and neutrophils, The most sensitive cells to all three MCPs are lymphocytes and monocytes, MCP-1 is a potent basophil activator but does not attract eosinophils, whereas, at higher concentrations, MCP-2 also stimulates both eosinophils and basophils, The signal transduction of MCPs on monocytes involves at least two G protein-linked C-C chemokine receptors: C-C CKR-1 binds MCP-3 and C-C CKR-2 binds MCP-1 and MCP-S but not MCP-2. Receptor binding leads to enhanced [Ca2+](i) for all chemokines except for MCP-2.