Preparation and isolation of the S‐carboxymethyl derivative chains of human fibrinogen
Preparation and isolation of the S‐carboxymethyl derivative chains of human fibrinogen
复制标题
人纤维蛋白原S-羧甲基衍生物链的制备与分离
DOI：
10.1016/0014-5793(71)80269-7
复制
发表时间：
1971
期刊：
影响因子：
3.5
通讯作者：
B. Blombäck
中科院分区：
文献类型：
--
作者：
G. Murano;B. Wiman;M. Blombäck;B. Blombäck
纤维蛋白原由三对多肽链组成：o (A)、/3 (B)和y[1,2]。纤维蛋白肽A和B分别被凝血酶从A (A)链和P (B)链上切割。最终产物是纤维蛋白[3,4]。纤维蛋白原的亚硫酸盐分解导致二硫桥[5]的断裂，并通过纸[51]，淀粉凝胶电泳[6]和柱层析[S， 71]分离得到S-亚砜链衍生物。最近，从牛[8]和人[9]纤维蛋白原中分离出s -硫链衍生物，利用[8]和梯度[9]醋酸钠缓冲体系在羧甲基纤维素上分离得到。在本研究中，3个人纤维蛋白原链的s -羧甲基衍生物在羧甲基纤维素上分离，在8 M尿素存在下，采用指数醋酸钠缓冲梯度和伴随的pH梯度。通过n端氨基酸分析、聚丙烯酰胺凝胶电泳、指纹图谱和氨基酸分析对每条链进行了表征和鉴定。
Fibrinogen is composed of three pairs of polypeptide chains: o (A),/3 (B), and y [1, 2]. Fibrinopeptides A and B are cleaved off the a (A) and P (B) chains respectively by thrombin. The resulting product is fibrin [3, 4]. Sulfitolysis of fibrinogen results in the cleavage of the disulfide bridges [5], and the resulting S-sulfo chain derivatives have been separated by paper [51, starch gel electrophoresis [6] and column chromatography [S, 71. More recently, the S-sulfo chain derivatives from bovine [8] and human [9] fibrinogen have been isolated on carboxymethyl cellulose using a stepwise [8] and gradient [9] sodium acetate buffer system. In this study, the S-carboxymethyl derivatives of the three human fibrinogen chains were separated on carboxymethyl cellulose, with an exponential sodium acetate buffer gradient, and a concomitant pH gradient, in the presence of 8 M urea. Each chain was characterized and identified by N-terminal amino acid analysis, polyacrylamide gel electrophoresis, fingerprinting, and amino acid analysis.