Coxsackievirus A16 in a 1-Day-Old Mouse Model of Central Nervous System Infection Shows Lower Neurovirulence than Enterovirus A71
Coxsackievirus A16 in a 1-Day-Old Mouse Model of Central Nervous System Infection Shows Lower Neurovirulence than Enterovirus A71
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DOI：
10.1016/j.jcpa.2020.02.001
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发表时间：
2020-04-01
影响因子：
0.8
通讯作者：
Wong, K. T.
中科院分区：
文献类型：
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作者：
Hooi, Y. T.;Ong, K. C.;Wong, K. T.
柯萨奇病毒A16型（CV-A16）和肠道病毒A71型（EV-A71）是引起幼儿手足口病的主要原因。尽管CV-A16的情况不那么严重，但这两种病毒都与严重的神经系统综合征有关，但它们的中枢神经系统感染之间的差异仍不清楚。我们使用临床分离的CV-A16和EV-A71毒株在1日龄小鼠模型中进行了比较感染研究，以更好地了解病毒的神经病理学和神经毒力。新的病毒型特异性原位杂交探针的开发和验证，以检测感染组织中的CV-A16和EV-A71 RNA。证明CV-A16病毒抗原/RNA，主要是在脑干和脊髓神经元，证实了神经毒力，但表现出较低的密度比EV-A71感染的动物。CV-A16的较高致死剂量（50）表明CV-A16的神经毒性较低。在前角白色物质和邻近的传出运动神经中的局灶性病毒抗原/RNA表明神经侵袭可能是通过周围运动神经中的逆行轴突运输。(C)2020爱思唯尔有限公司保留所有权利。
Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) are the major causes of hand, foot and mouth disease in young children. Although less so with CV-A16, both viruses are associated with serious neurological syndromes, but the differences between their central nervous system infections remain unclear. We conducted a comparative infection study using clinically-isolated CV-A16 and EV-A71 strains in a 1-day-old mouse model to better understand the neuropathology and neurovirulence of the viruses. New serotype-specific probes for in situ hybridization were developed and validated to detect CV-A16 and EV-A71 RNA in infected tissues. Demonstration of CV-A16 virus antigens/RNA, mainly in the brainstem and spinal cord neurons, confirmed neurovirulence, but showed lower densities than in EV-A71 infected animals. A higher lethal dose(50) for CV-A16 suggested that CV-A16 is less neurovirulent. Focal virus antigens/RNA in the anterior horn white matter and adjacent efferent motor nerves suggested that neuroinvasion is possibly via retrograde axonal transport in peripheral motor nerves. (C) 2020 Elsevier Ltd. All rights reserved.