Crystal structure of the extracellular domain of the receptor-like kinase TMK3 from Arabidopsis thaliana
Crystal structure of the extracellular domain of the receptor-like kinase TMK3 from Arabidopsis thaliana
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拟南芥受体样激酶 TMK3 胞外结构域的晶体结构
DOI：
10.1107/s2053230x20010122
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发表时间：
2020-08-01
影响因子：
0.9
通讯作者：
Ming, Zhenhua
中科院分区：
文献类型：
--
作者：
Chen, Hong;Kong, Yanqiong;Ming, Zhenhua
跨膜激酶（TMK）是植物受体样激酶（RLK）家族的成员。 TMK的特征是细胞外亮氨酸重复（LRR）结构域，单个跨膜区域和细胞质激酶结构域。 TMK已被证明充当细胞膨胀和细胞增殖的关键调节剂。在这里，TMK3（TMK3-ECD）的细胞外结构域的晶体结构确定为2.06 Angstrom的分辨率，RWOWS为17.69％，Re-free R-Free为20.58％。与TMK1的细胞外结构域相似，TMK3-ECD结构包含两个螺线管，其中第十和11个LRR之间具有13个LRR和一个非LRR区域（316-364）。 TMK3-ECD与包含非LRR区域的其他LRR-RLK的比较表明，非LRR区域在结构完整性中起关键作用，并且可能有助于配体相互作用。 TMK3-ECD的非LRR区域的特征是两个可能具有关键生物学意义的二硫键。
Transmembrane kinases (TMKs) are members of the plant receptor-like kinase (RLK) family. TMKs are characterized by an extracellular leucine-rich-repeat (LRR) domain, a single transmembrane region and a cytoplasmic kinase domain. TMKs have been shown to act as critical modulators of cell expansion and cell proliferation. Here, the crystal structure of the extracellular domain of TMK3 (TMK3-ECD) was determined to a resolution of 2.06 angstrom, with an R-work of 17.69% and an R-free of 20.58%. Similar to the extracellular domain of TMK1, the TMK3-ECD structure contains two solenoids with 13 LRRs and a non-LRR region (316-364) between the tenth and 11th LRRs. A comparison of TMK3-ECD with other LRR-RLKs that contain a non-LRR region indicates that the non-LRR region plays a critical role in structural integrity and may contribute to ligand interactions. The non-LRR region of TMK3-ECD is characterized by two disulfide bonds that may have critical biological implications.