Initial differences in lipid processing leading to pig- and beef- derived mature adipocyte dedifferentiation
Initial differences in lipid processing leading to pig- and beef- derived mature adipocyte dedifferentiation
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DOI：
10.7939/r3zs2ks7z
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Jie Chen;M. Guridi;G. Hausman;M. Dodson;R. B. Russell
中科院分区：
文献类型：
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作者：
Jie Chen;M. Guridi;G. Hausman;M. Dodson;R. B. Russell
猪源性成熟脂肪细胞的克隆培养物能够在体外去分化并形成具有增殖能力的子代细胞。最初的脂质加工与在牛肉来源的脂肪细胞培养物中观察到的不同。成熟的猪脂肪细胞在增殖前排出脂质，而牛肉来源的脂肪细胞对称或不对称地分裂而不排出脂质。这些观察结果表明，成熟脂肪细胞的去分化依赖于物种特异性机制，或者猪源脂肪细胞需要不同的培养条件以类似于牛肉脂肪细胞的方式去分化。该体外系统将有助于我们了解脂质代谢、单细胞的调节、细胞去分化的过程和/或脂肪组织中假定的干细胞的特征。
Clonal cultures of pig-derived mature adipocytes are capable of dedifferentiating and forming proliferative-competent progeny cells in vitro. Initial lipid processing, is different to that observed in cultures of beef-derived adipocytes. Mature pig adipocytes extrude lipid before proliferation, whereas beef-derived adipocytes symmetrically, or asymmetrically, divide without expelling lipid. These observations suggest that dedifferentiation of mature adipocytes relies on species-specific mechanisms, or that different culture conditions are required for pigderived adipocytes to dedifferentiate in a manner similar to beef adipocytes. This in vitro system will aid in our understanding of lipid metabolism, regulation of single cells, processes involved in dedifferentiation of cells, and/or characteristics of putative stem cells residing in adipose tissue.