Expression of HGF and IGF-1 during regeneration of masseter muscle in mdx mice
Expression of HGF and IGF-1 during regeneration of masseter muscle in mdx mice
复制标题
DOI：
10.1007/s10974-010-9209-6
复制
发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Ide, Yoshinobu
中科院分区：
文献类型：
--
作者：
Honda, Hidemitsu;Abe, Shinichi;Ide, Yoshinobu
本研究研究了生长因子HGF和IGF-1在mdx小鼠肌肉再生过程中的表达。据报道，HGF和IGF-1在体外肌肉组织再生过程中表达。然而，很少有研究关注HGF和IGF-1在mdx小鼠肌肉再生中的作用，mdx缺乏肌营养不良蛋白基因的表达。在本研究中，我们使用组织学分析、免疫组织化学和Western blotting检测mdx和B10（对照）小鼠肌肉再生过程中咬肌中HGF和IGF-1的表达，并通过RT-PCR检测基因表达，时间分别为3、4和9周。Mdx小鼠在3周和4周时再生肌细胞细胞质中出现局部HGF和IGF-1阳性，但在9周时几乎没有明显的反应性。对照组在任何检查时间点IGF-1均为完全阴性。Western blot结果显示，3周和4周时，mdx小鼠的HGF和IGF-1表达强于B10小鼠，但9周时，两组均无表达。RT-PCR也得到了类似的结果。这些目前的结果表明，HGF和IGF-1似乎在mdx小鼠咬肌再生中起重要作用。
This study investigated the expression of the growth factors HGF and IGF-1 during the process of muscle regeneration in mdx mice. HGF and IGF-1 are reportedly expressed during the regeneration of muscle tissue in vitro. However, few studies have focused on the role of HGF and IGF-1 during muscle regeneration in mdx mice, which lack expression of the dystrophin gene. In the present study, we examined the expression of HGF and IGF-1 in masseter muscle during muscle regeneration in mdx and B10 (control) mice using histological analysis, immunohistochemistry and Western blotting, as well as examining gene expression by RT-PCR, at 3, 4 and 9 weeks. Mdx mice showed localized HGF and IGF-1 positivity in the cytoplasm of regenerating muscle cells at 3 and 4 weeks, but hardly any reactivity was evident at 9 weeks. The control group was completely negative for IGF-1 at any of the examined time points. Western blotting showed stronger expression of HGF and IGF-1 in mdx mice than in B10 mice at 3 and 4 weeks, but at 9 weeks the expression was absent in both groups. Similar results were obtained using RT-PCR. These present results suggest that HGF and IGF-1 appear to play an important role during regeneration of the masseter muscle in mdx mice.