Glycolipid expression in prostatic tissue and analysis of the antigen recognized by antiprostatic monoclonal antibody APG1.
Glycolipid expression in prostatic tissue and analysis of the antigen recognized by antiprostatic monoclonal antibody APG1.
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前列腺组织中糖脂的表达及抗前列腺单克隆抗体APG1识别的抗原分析。
作者：
M. Satoh;Y. Fukushi;S. Kawamura;C. Ohyama;S. Saito;S. Orikasa;E. Nudleman;S. Hakamori
观察糖脂在前列腺增生、前列腺癌和正常前列腺组织中的表达模式。进一步分析了小鼠单克隆抗体APG 1识别的抗原，该抗体是通过免疫从人前列腺癌中提取的糖脂获得的。在癌组织中，发现乳糖基和globoside系列糖脂都普遍减少，尽管在神经节苷脂系列中，GM 3和GD 3没有减少，并且发现仅具有比GD 2更长链的糖脂减少。这些结果表明，糖链延长的抑制，而不是唾液酸化，是人前列腺癌变过程中发生的主要合成变化。APG 1在薄层层析板上仅与神经节苷脂组分的GM 2和GD 2附近的两个条带反应，但它不与包括GM 2和GD 2在内的任何已知的神经节苷脂系列的神经节苷脂反应。组织化学上，APG 1仅在人前列腺冰冻组织切片中显示强烈反应，且反应性随肿瘤级别的升高而降低。因此，该抗原被认为是与非神经节苷脂系列神经节苷脂反应的前列腺特异性和分化抗原。
The expression patterns of glycolipid from prostatic hyperplasia, prostatic cancer and normal prostate tissue were observed. A further analysis of antigen recognized by mouse monoclonal antibody APG1, which was gained by immunizing glycolipids extracted from human prostate cancer, was also performed. In cancer tissue, both of the lactosyl and globoside series glycolipids were found to be generally reduced, although in the ganglioside series, GM3 and GD3 were not reduced and only the glycolipids with longer chains than GD2 were found to be reduced. These results indicated that the inhibition of sugar chain elongation, but not sialylation, was the main synthetic change occurring with carcinogenesis of the human prostate. APG1 reacted with only two bands near GM2 and GD2 of the ganglioside fraction on a thin-layer chromatography plate, but it did not react with any of the known gangliosides of the ganglioside series including GM2 and GD2. Histochemically, APG1 showed intense reaction only in frozen tissue sections of human prostate, and the reactivity decreased with the increasing grade of cancer. Therefore, this antigen was considered to be a prostate-specific and differentiated antigen reacting with nonganglioseries gangliosides.