SARS-associated coronavirus replication in cell lines.
SARS-associated coronavirus replication in cell lines.
复制标题
DOI：
10.3201/eid1201.050496
复制
发表时间：
2006-01
影响因子：
11.8
通讯作者：
Kaye M
中科院分区：
文献类型：
--
作者：
Kaye M
病毒可在几种常见细胞系中复制，有时无细胞病变效应。鉴于实验室相关的严重急性呼吸综合征相关冠状病毒（SARS-CoV）感染的可能性，我们必须知道哪些细胞系对该病毒易感。我们研究了21种常规用于病毒分离或研究的细胞系。在感染SARS-CoV后，观察细胞的细胞病变效应，并使用定量实时聚合酶链反应来测量正在进行的病毒复制。间接免疫荧光试验也用作确证试验。该研究确定了10个新的细胞系能够支持SARS-CoV的复制，并证实了先前报道的4个细胞系的易感性。这项研究表明，SARS-CoV可以在几种通常用于诊断或研究目的的细胞系中分离出来。它还表明，SARS-CoV可以在几种细胞系中达到高滴度，有时在没有特异性细胞病变效应的情况下。
Virus can replicate in several common cell lines, sometimes without cytopathic effect. Given the potential for laboratory-associated severe acute respiratory syndrome–associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.