Genome-Wide Identification and Characterization of Hsf and Hsp Gene Families and Gene Expression Analysis under Heat Stress in Eggplant (Solanum melongema L.)
Genome-Wide Identification and Characterization of Hsf and Hsp Gene Families and Gene Expression Analysis under Heat Stress in Eggplant (Solanum melongema L.)
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茄子 (Solanum melongema L.) Hsf 和 Hsp 基因家族的全基因组鉴定和表征以及热应激下的基因表达分析
DOI：
10.3390/horticulturae7060149
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发表时间：
2021-06-01
期刊：
影响因子：
3.1
通讯作者：
Sun, Baojuan
中科院分区：
文献类型：
--
作者：
Gong, Chao;Pang, Qiangqiang;Sun, Baojuan
在高温胁迫下，植物细胞中的大量蛋白质会变性、失活。同时会快速诱导Hsfs和Hsps去除变性蛋白质，从而避免细胞程序性死亡，从而增强植物的耐热性。本研究对热胁迫下茄子中的Hsf和Hsp基因家族进行了全面的鉴定和分析。使用来自拟南芥的内源基因从茄子基因组中鉴定出总共 24 个 Hsf 样基因和 117 个 Hsp 样基因。茄子各亚科Hsf和Hsp基因的基因结构和基序组成相对保守。 RNA-seq数据和qRT-PCR分析表明，大多数茄子Hsf和Hsp基因的表达在暴露于热胁迫后增加，特别是在耐热品系中。综合分析表明，特定SmHsfs基因的下游涉及不同组的SmHsps基因。这些结果为揭示SmHsps和SmHsp在茄子耐热性中的作用提供了基础，这可能有助于了解茄子中SmHsps和SmHsp的耐热机制。
Under high temperature stress, a large number of proteins in plant cells will be denatured and inactivated. Meanwhile Hsfs and Hsps will be quickly induced to remove denatured proteins, so as to avoid programmed cell death, thus enhancing the thermotolerance of plants. Here, a comprehensive identification and analysis of the Hsf and Hsp gene families in eggplant under heat stress was performed. A total of 24 Hsf-like genes and 117 Hsp-like genes were identified from the eggplant genome using the interolog from Arabidopsis. The gene structure and motif composition of Hsf and Hsp genes were relatively conserved in each subfamily in eggplant. RNA-seq data and qRT-PCR analysis showed that the expressions of most eggplant Hsf and Hsp genes were increased upon exposure to heat stress, especially in thermotolerant line. The comprehensive analysis indicated that different sets of SmHsps genes were involved downstream of particular SmHsfs genes. These results provided a basis for revealing the roles of SmHsps and SmHsp for thermotolerance in eggplant, which may potentially be useful for understanding the thermotolerance mechanism involving SmHsps and SmHsp in eggplant.