CHANGES IN THE MECHANISM OF CA-2+ MOBILIZATION DURING THE DIFFERENTIATION OF BC3H1 MUSCLE-CELLS
CHANGES IN THE MECHANISM OF CA-2+ MOBILIZATION DURING THE DIFFERENTIATION OF BC3H1 MUSCLE-CELLS
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DOI：
10.1042/bj2730219
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发表时间：
1991-01-01
影响因子：
4.1
通讯作者：
BORGHGRAEF, R
中科院分区：
文献类型：
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作者：
DESMEDT, H;PARYS, JB;BORGHGRAEF, R
BC3H1肌细胞中的Ca 2+螯合和释放强烈依赖于分化阶段。 在增殖细胞中，超过90%的螯合Ca 2+是Ins（1，4，5）P3敏感的，25%是咖啡因敏感的。 在分化的细胞中，Ca2+的积累是5倍高，InsP3不敏感，但约60%的螯合Ca2+是咖啡因敏感的。 这些变化在添加生长刺激物后是可逆的。 同样，通过测量单个完整BC 3 H1细胞的细胞内Ca 2+浓度，发现在肌肉细胞分化期间，组胺反应细胞的数量减少，咖啡因反应细胞的数量增加。 这些数据表明，在BC3H1成肌细胞的肌肉表型的发展诱导的Ca2+动员机制的主要重排。
Ca2+ sequestration and release in BC3H1 muscle cells is strongly dependent on the stage of differentiation. In proliferating cells, more than 90% of the sequestered Ca2+ was Ins(1,4,5) P3-sensitive and 25% was caffeine-sensitive. In differentiated cells, the Ca2+ accumulation was 5-fold higher and was InsP3-insensitive, but about 60% of the sequestered Ca2+ was caffeine-sensitive. These changes were reversible upon addition of growth stimuli. Similarly, by measuring the intracellular Ca2+ concentration in single intact BC3H1 cells, it was found that the number of histamine-responsive cells decreased and the number of caffeine-responsive cells increased during muscle cell differentiation. These data indicate that the development of the muscle phenotype in BC3H1 myoblasts induces a major rearrangement of the mechanisms for Ca2+ mobilization.