Hydrophilic Interaction Electrokinetic Chromatography Using Bio-based Nanofillers
Hydrophilic Interaction Electrokinetic Chromatography Using Bio-based Nanofillers
复制标题
使用生物基纳米填料的亲水相互作用电动色谱法
DOI：
10.1002/elps.201300558
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发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Koji Otsuka
中科院分区：
文献类型：
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作者：
Takayuki Kawai;Masato Watanabe;Kojiro Uetani;Yudai Fukushima;Kenji Sueyoshi;Takuya Kubo;Fumihiko Kitagawa;Hiroyuki Yano;Koji Otsuka
基于亲水相互作用（HI）的分离（如HILIC）可有效分析亲水性生物样品，如碳水化合物、肽和代谢物。为了克服传统HILIC消耗大量有机溶剂和分离柱容易劣化的缺点，我们开发了采用生物基纳米材料作为亲水性假固定相的HI电动色谱（EKC）。通过机械/化学处理，将纤维素、甲壳素和壳聚糖加工成10 nm宽的纳米纤维/纳米晶须（NF/NW），其分别长于/短于1000/200 nm。在使用0.001%不带电纤维素NF的寡糖的HI-EKC中，观察到大尺寸寡糖的强相互作用，保留因子（k）高达1.56，表明HILIC模式相互作用。在含有0.1%带正电荷的壳聚糖NF的HI-EKC中，苯二磺酸、苯磺酸（BS）和对羟基BS（HBS）的k值分别为0.036、0.018和0.018，表明离子交换作用主要通过磺酸根基团发生。最后，使用0.05%几丁质或壳聚糖NW证明了HI-EKC。在使用甲壳素和壳聚糖纳米线的两种情况下，与k < 0.070的BS相比，HBS显示出更强的相互作用，k> 0.192。这表明NF和NW之间的结构差异影响了离子交换和HILIC模式方面的HI行为。
Hydrophilic interaction (HI)‐based separation like HILIC is effective for analyzing hydrophilic biological samples such as carbohydrates, peptides, and metabolites. To overcome the drawbacks of conventional HILIC such as large consumption of organic solvents and easy deterioration of the separation column, we developed HI electrokinetic chromatography (EKC) by employing bio‐based nanomaterials as the hydrophilic pseudostationary phase. By mechanical/chemical treatments, cellulose, chitin, and chitosan were processed to 10‐nm wide nanofibers/nanowhiskers (NFs/NWs), which are longer/shorter than 1000/200 nm, respectively. In HI‐EKC of oligosaccharides using 0.001% uncharged cellulose NFs, strong interaction was observed for the large‐size oligosaccharides with the retention factors (k) of up to 1.56, indicating a HILIC‐mode interaction. In HI‐EKC with 0.1% positively charged chitosan NFs, benzenedisulfonic acid, benzenesulfonic acid (BS), andp‐hydroxy BS (HBS) hadkvalues of 0.036, 0.018, and 0.018, respectively, suggesting that the ion‐exchange interaction mainly occurred via sulfonate groups. Finally, HI‐EKC was demonstrated using 0.05% chitin or chitosan NWs. In both cases using chitin and chitosan NWs, HBS showed much stronger interaction withk> 0.192 compared with BS withk< 0.070. It indicated structural difference between NFs and NWs affected the HI behavior in terms of both the ion‐exchange and HILIC modes.