TRANSCRIPTION OF GLNA BY PURIFIED ESCHERICHIA-COLI COMPONENTS - CORE RNA-POLYMERASE AND THE PRODUCTS OF GLNF, GLNG, AND GLNL
TRANSCRIPTION OF GLNA BY PURIFIED ESCHERICHIA-COLI COMPONENTS - CORE RNA-POLYMERASE AND THE PRODUCTS OF GLNF, GLNG, AND GLNL
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DOI：
10.1073/pnas.82.24.8453
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
MAGASANIK, B
中科院分区：
文献类型：
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作者：
HUNT, TP;MAGASANIK, B
我们已经证明大肠杆菌纯化的glnF （ntrA）产物与核心RNA聚合酶结合。这些蛋白一起在超螺旋模板上的氮调控启动子glnAp2上启动转录。glnAp2在线性模板上的转录起始还需要glnG的产物NRI （ntrC）和glnL突变等位基因NRII2302 （ntnB）的产物。这些结果确定glnF产物为新的。sigma。氮调控启动子和固氮启动子转录所特别需要的因子。我们建议rpoN作为glnF和。sigma的适当名称。60美元的产品。我们的结果表明。sigma。60RNA聚合酶识别氮调控/固氮启动子共识序列C-T-G-G-Y-A-Y-R-N4-T-T-G-C-A。完整细胞的转录起始似乎还需要GlnG产物NRI的活性形式。NRI向活性形态的转化显然是由glnL的产物NRII对氮剥夺的反应引起的。
We have shown that the purified glnF (ntrA) product of Escherichia coli binds to core RNA polymerase. Together these proteins initiated transcription at the nitrogen-regulated promoter glnAp2 on a supercoiled template. The initiation of transcription at glnAp2 on a linear template required in addition NRI, the product of glnG (ntrC), and NRII2302, the product of a mutant allele of glnL (ntnB). These results identify the glnF product as a new .sigma. factor specifically required for the transcription of nitrogen-regulated and of nitrogen-fixation promoters. We propose rpoN as the proper designation for glnF, and .sigma.60 for its product. Our results indicate that .sigma.60 RNA polymerase recognizes the nitrogen-regulated/nitrogen-fixation promoter consensus sequence C-T-G-G-Y-A-Y-R-N4-T-T-G-C-A. Initiation of transcription in the intact cell appears to require in addition the active form of NRI, the product of GlnG. Conversion of NRI to its active form is apparently brought about by NRII, the product of glnL, in response to nitrogen deprivation.